Literature DB >> 173296

A pitfall in the interpretation of data on ligand-protein interaction.

S Swillens, J E Dumont.   

Abstract

Binding reactions are usually investigated by separation of free and bound labelled ligand. Underestimation of the amount bound leads to inaccurate estimation of the dissociation constant and to wrong conclusions on the validity of the model of binding. The activation of cyclic AMP-dependent protein kinase is treated as an example.

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Year:  1975        PMID： 173296      PMCID： PMC1165687          DOI： 10.1042/bj1490779

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  6 in total

1.  Protein kinase and cyclic 3',5'-AMP: significance of binding and activation constants.

Authors:  S Swillens; E Van Cauter; J E Dumont
Journal:  Biochim Biophys Acta       Date:  1974-10-17

2.  Further studies on the properties of the rabbit reticulocyte adenosine 3',5'-cyclic monophosphate-dependent protein kinase I.

Authors:  M Tao
Journal:  Arch Biochem Biophys       Date:  1971-03       Impact factor: 4.013

3.  Purification and properties of rabbit skeletal muscle adenosine 3',5'-monophosphate-dependent protein kinases.

Authors:  E M Reimann; D A Walsh; E G Krebs
Journal:  J Biol Chem       Date:  1971-04-10       Impact factor: 5.157

4.  An assay for adenosine 3',5'-cyclic monophosphate based on the association of the nucleotide with a partially purified binding protein.

Authors:  G M Walton; L D Garren
Journal:  Biochemistry       Date:  1970-10-13       Impact factor: 3.162

5.  Role of the receptor in the mechanism of action of adenosine 3':5'-cyclic monophosphate.

Authors:  G N Gill; L D Garren
Journal:  Proc Natl Acad Sci U S A       Date:  1971-04       Impact factor: 11.205

6.  A protein binding assay for adenosine 3':5'-cyclic monophosphate.

Authors:  A G Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1970-09       Impact factor: 11.205
  6 in total
  4 in total

1.  Equilibrium-dialysis studies of the interaction between cholic acid and 100000g-supernatant preparations from the rat liver.

Authors:  R C Strange; I A Nimmo; I W Percy-Robb
Journal:  Biochem J       Date:  1976-05-15       Impact factor: 3.857

2.  An evaluation of ways of using equilibrium dialysis to quantify the binding of ligand to macromolecule.

Authors:  I A Nimmo; G L Atkins; R C Strange; I W Percy-Robb
Journal:  Biochem J       Date:  1977-07-01       Impact factor: 3.857

3.  Factors affecting the binding of [3H]adenosine 3':5'-cyclic monophosphate to protein kinase from bovine adrenal cortex.

Authors:  S O Doskeland; P M Ueland; H J Haga
Journal:  Biochem J       Date:  1977-03-01       Impact factor: 3.857

4.  Probing the structure-function relationship of nerve growth factor.

Authors:  S Vroegop; D Decker; J Hinzmann; R Poorman; S Buxser
Journal:  J Protein Chem       Date:  1992-02
  4 in total

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