Literature DB >> 17329447

Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa.

Amalia S Magaret1, Anna Wald, Meei-Li Huang, Stacy Selke, Larry Corey.   

Abstract

In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.

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Year:  2007        PMID： 17329447      PMCID： PMC1865863          DOI： 10.1128/JCM.01405-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  Evaluation of a quantitative competitive PCR assay for measuring herpes simplex virus DNA content in genital tract secretions.

Authors:  A Hobson; A Wald; N Wright; L Corey
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

2.  Reactivation of genital herpes simplex virus type 2 infection in asymptomatic seropositive persons.

Authors:  A Wald; J Zeh; S Selke; T Warren; A J Ryncarz; R Ashley; J N Krieger; L Corey
Journal:  N Engl J Med       Date:  2000-03-23       Impact factor: 91.245

3.  Polymerase chain reaction for detection of herpes simplex virus (HSV) DNA on mucosal surfaces: comparison with HSV isolation in cell culture.

Authors:  Anna Wald; Meei-Li Huang; David Carrell; Stacy Selke; Lawrence Corey
Journal:  J Infect Dis       Date:  2003-10-31       Impact factor: 5.226

4.  Frequent reactivation of herpes simplex virus among HIV-1-infected patients treated with highly active antiretroviral therapy.

Authors:  Christine M Posavad; Anna Wald; Steven Kuntz; Meei Li Huang; Stacy Selke; Elizabeth Krantz; Lawrence Corey
Journal:  J Infect Dis       Date:  2004-07-13       Impact factor: 5.226

5.  Herpes simplex virus shedding among human immunodeficiency virus-negative men who have sex with men: site and frequency of shedding.

Authors:  M R Krone; S R Tabet; M Paradise; A Wald; L Corey; C L Celum
Journal:  J Infect Dis       Date:  1998-10       Impact factor: 5.226

6.  Valacyclovir and acyclovir for suppression of shedding of herpes simplex virus in the genital tract.

Authors:  Rachna Gupta; Anna Wald; Elizabeth Krantz; Stacy Selke; Terri Warren; Mauricio Vargas-Cortes; Gerri Miller; Lawrence Corey
Journal:  J Infect Dis       Date:  2004-09-20       Impact factor: 5.226

7.  Genital shedding of herpes simplex virus among men.

Authors:  Anna Wald; Judith Zeh; Stacy Selke; Terri Warren; Rhoda Ashley; Lawrence Corey
Journal:  J Infect Dis       Date:  2002-10-15       Impact factor: 5.226

8.  Once-daily valacyclovir to reduce the risk of transmission of genital herpes.

Authors:  Lawrence Corey; Anna Wald; Raj Patel; Stephen L Sacks; Stephen K Tyring; Terri Warren; John M Douglas; Jorma Paavonen; R Ashley Morrow; Karl R Beutner; Leonid S Stratchounsky; Gregory Mertz; Oliver N Keene; Helen A Watson; Dereck Tait; Mauricio Vargas-Cortes
Journal:  N Engl J Med       Date:  2004-01-01       Impact factor: 91.245

9.  Frequency of symptomatic and asymptomatic herpes simplex virus type 2 reactivations among human immunodeficiency virus-infected men.

Authors:  T Schacker; J Zeh; H L Hu; E Hill; L Corey
Journal:  J Infect Dis       Date:  1998-12       Impact factor: 5.226

10.  Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples.

Authors:  A J Ryncarz; J Goddard; A Wald; M L Huang; B Roizman; L Corey
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948
  10 in total
  63 in total

1.  Valacyclovir suppressive therapy reduces plasma and breast milk HIV-1 RNA levels during pregnancy and postpartum: a randomized trial.

Authors:  Alison L Drake; Alison C Roxby; Francisca Ongecha-Owuor; James Kiarie; Grace John-Stewart; Anna Wald; Barbra A Richardson; Jane Hitti; Julie Overbaugh; Sandra Emery; Carey Farquhar
Journal:  J Infect Dis       Date:  2011-12-06       Impact factor: 5.226

2.  Rapid polymerase chain reaction assay to detect herpes simplex virus in the genital tract of women in labor.

Authors:  Carolyn Gardella; Meei-Li Huang; Anna Wald; Amalia Magaret; Stacy Selke; Rhoda Morrow; Lawrence Corey
Journal:  Obstet Gynecol       Date:  2010-06       Impact factor: 7.661

3.  Development and Validation of a Laboratory-Developed Multiplex Real-Time PCR Assay on the BD Max System for Detection of Herpes Simplex Virus and Varicella-Zoster Virus DNA in Various Clinical Specimens.

Authors:  Sylvie Pillet; Paul O Verhoeven; Amélie Epercieux; Thomas Bourlet; Bruno Pozzetto
Journal:  J Clin Microbiol       Date:  2015-04-15       Impact factor: 5.948

4.  Persistent genital herpes simplex virus-2 shedding years following the first clinical episode.

Authors:  Warren Phipps; Misty Saracino; Amalia Magaret; Stacy Selke; Mike Remington; Meei-Li Huang; Terri Warren; Corey Casper; Lawrence Corey; Anna Wald
Journal:  J Infect Dis       Date:  2010-12-09       Impact factor: 5.226

5.  Immunization with a vaccine combining herpes simplex virus 2 (HSV-2) glycoprotein C (gC) and gD subunits improves the protection of dorsal root ganglia in mice and reduces the frequency of recurrent vaginal shedding of HSV-2 DNA in guinea pigs compared to immunization with gD alone.

Authors:  Sita Awasthi; John M Lubinski; Carolyn E Shaw; Shana M Barrett; Michael Cai; Fushan Wang; Michael Betts; Susan Kingsley; Daniel J Distefano; John W Balliet; Jessica A Flynn; Danilo R Casimiro; Janine T Bryan; Harvey M Friedman
Journal:  J Virol       Date:  2011-08-03       Impact factor: 5.103

6.  Oral and Vaginal Tenofovir for Genital Herpes Simplex Virus Type 2 Shedding in Immunocompetent Women: A Double-Blind, Randomized, Cross-over Trial.

Authors:  Rachel A Bender Ignacio; Tara Perti; Amalia S Magaret; Sharanya Rajagopal; Claire E Stevens; Meei-Li Huang; Stacy Selke; Christine Johnston; Jeanne Marrazzo; Anna Wald
Journal:  J Infect Dis       Date:  2015-06-04       Impact factor: 5.226

7.  Plasma and cerebrospinal fluid herpes simplex virus levels at diagnosis and outcome of neonatal infection.

Authors:  Ann J Melvin; Kathleen M Mohan; Joshua T Schiffer; Linda M Drolette; Amalia Magaret; Lawrence Corey; Anna Wald
Journal:  J Pediatr       Date:  2014-12-06       Impact factor: 4.406

8.  Virologic and immunologic evidence of multifocal genital herpes simplex virus 2 infection.

Authors:  Christine Johnston; Jia Zhu; Lichen Jing; Kerry J Laing; Christopher M McClurkan; Alexis Klock; Kurt Diem; Lei Jin; Jeffrey Stanaway; Elizabeth Tronstein; William W Kwok; Meei-Li Huang; Stacy Selke; Youyi Fong; Amalia Magaret; David M Koelle; Anna Wald; Lawrence Corey
Journal:  J Virol       Date:  2014-02-19       Impact factor: 5.103

9.  Increase in HSV shedding at initiation of antiretroviral therapy and decrease in shedding over time on antiretroviral therapy in HIV and HSV-2 infected persons.

Authors:  Emily S Ford; Amalia S Magaret; Cedric W Spak; Stacy Selke; Steve Kuntz; Lawrence Corey; Anna Wald
Journal:  AIDS       Date:  2018-11-13       Impact factor: 4.177

10.  Consistent viral DNA quantification after prolonged storage at ambient temperature.

Authors:  Benjamin Zaniello; Meei-Li Huang; Anqi Cheng; Stacy Selke; Anna Wald; Keith R Jerome; Amalia S Magaret
Journal:  J Virol Methods       Date:  2015-11-22       Impact factor: 2.014
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