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Literature DB >> 17303564

Stabilizing effect of Zn2+ in native bovine rhodopsin.

Paul S-H Park¹, K Tanuj Sapra, Michał Koliński, Sławomir Filipek, Krzysztof Palczewski, Daniel J Muller.

Abstract

Single-molecule force spectroscopy (SMFS) is a powerful tool to dissect molecular interactions that govern the stability and function of proteins. We applied SMFS to understand the effect of Zn2+ on the molecular interactions underlying the structure of rhodopsin. Force-distance curves obtained from SMFS assays revealed the strength and location of molecular interactions that stabilize structural segments within this receptor. The inclusion of ZnCl2 in SMFS assay buffer increased the stability of most structural segments. This effect was not mimicked by CaCl2, CdCl2, or CoCl2. Thus, Zn2+ stabilizes the structure of rhodopsin in a specific manner.

Entities: Chemical Gene Species

Mesh：

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Year: 2007 PMID： 17303564 PMCID： PMC2043472 DOI： 10.1074/jbc.M610341200

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

52 in total

1. The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure.

Authors: Tetsuji Okada; Minoru Sugihara; Ana-Nicoleta Bondar; Marcus Elstner; Peter Entel; Volker Buss
Journal: J Mol Biol Date: 2004-09-10 Impact factor: 5.469

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Journal: Int Rev Neurobiol Date: 1989 Impact factor: 3.230

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5. Histochemical localization of zinc and copper in rat ocular tissues.

Authors: Y Hirayama
Journal: Acta Histochem Date: 1990 Impact factor: 2.479

6. Structure of bovine rhodopsin in a trigonal crystal form.

Authors: Jade Li; Patricia C Edwards; Manfred Burghammer; Claudio Villa; Gebhard F X Schertler
Journal: J Mol Biol Date: 2004-11-05 Impact factor: 5.469

7. Critical role of transmembrane segment zinc binding in the structure and function of rhodopsin.

Authors: Aleksandar Stojanovic; Jeremiah Stitham; John Hwa
Journal: J Biol Chem Date: 2004-06-12 Impact factor: 5.157

8. Refinement of a homology model of the mu-opioid receptor using distance constraints from intrinsic and engineered zinc-binding sites.

Authors: Carol B Fowler; Irina D Pogozheva; Harry LeVine; Henry I Mosberg
Journal: Biochemistry Date: 2004-07-13 Impact factor: 3.162

9. Identification of core amino acids stabilizing rhodopsin.

Authors: A J Rader; Gülsüm Anderson; Basak Isin; H Gobind Khorana; Ivet Bahar; Judith Klein-Seetharaman
Journal: Proc Natl Acad Sci U S A Date: 2004-05-03 Impact factor: 11.205

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Authors: Z A Karcioglu
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24 in total

1. Structural, energetic, and mechanical perturbations in rhodopsin mutant that causes congenital stationary night blindness.

Authors: Shiho Kawamura; Alejandro T Colozo; Lin Ge; Daniel J Müller; Paul S-H Park
Journal: J Biol Chem Date: 2012-05-01 Impact factor: 5.157

Stabilizing effect of Zn2+ in native bovine rhodopsin.

1. The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure.

Review 2. Neurobiology of zinc and zinc-containing neurons.

3. Release of endogenous Zn2+ from brain tissue during activity.

4. Preparation of retinal rod outer segments.

5. Histochemical localization of zinc and copper in rat ocular tissues.

6. Structure of bovine rhodopsin in a trigonal crystal form.

7. Critical role of transmembrane segment zinc binding in the structure and function of rhodopsin.

8. Refinement of a homology model of the mu-opioid receptor using distance constraints from intrinsic and engineered zinc-binding sites.

9. Identification of core amino acids stabilizing rhodopsin.

Review 10. Zinc in the eye.

1. Structural, energetic, and mechanical perturbations in rhodopsin mutant that causes congenital stationary night blindness.

2. Conformational stability of hepatitis C virus NS3 protease.

3. Conservation of molecular interactions stabilizing bovine and mouse rhodopsin.

Review 4. Vertebrate membrane proteins: structure, function, and insights from biophysical approaches.

5. Point mutations in membrane proteins reshape energy landscape and populate different unfolding pathways.

6. Mechanical properties of bovine rhodopsin and bacteriorhodopsin: possible roles in folding and function.

7. Role of extracellular glutamic acids in the stability and energy landscape of bacteriorhodopsin.

8. Kinetic, energetic, and mechanical differences between dark-state rhodopsin and opsin.

9. Salt effects on the conformational stability of the visual G-protein-coupled receptor rhodopsin.

10. Cholesterol increases kinetic, energetic, and mechanical stability of the human β2-adrenergic receptor.