| Literature DB >> 1730289 |
A Hashimoto1, T Nishikawa, T Hayashi, N Fujii, K Harada, T Oka, K Takahashi.
Abstract
Free amino acid enantiomers in adult rat brain extracts were analyzed as their N,O-pentafluoropropionyl isopropyl derivatives by gas chromatography on a capillary column of Chirasil-L-Val. A peak X, which exhibited the same retention time as the N,O-pentafluoropropionyl isopropyl derivative of authentic D-serine, was detected in the brain extracts. Electron impact and positive chemical ionization mass spectra of the peak X of the brain extracts were identical to those of authentic D-serine. The concentration of free D-serine and the ratio of D-serine/total serine in the brain were estimated to be 0.27 and 0.23 mumol/g of wet weight, respectively. These data provide the first evidence that substantial quantities of free D-serine are present in mammalian brain tissues.Entities:
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Year: 1992 PMID： 1730289 DOI： 10.1016/0014-5793(92)80397-y
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124