| Literature DB >> 17292402 |
Jaby Jacob1, Robin S Dothager, P Thiyagarajan, Tobin R Sosnick.
Abstract
The dimensions of a denatured protein, fully reduced ribonuclease A (r-RNase A), have been measured using synchrotron-based small angle X-ray scattering. The radius of gyration, 34-35 A, is unchanged from 0-6 M guanidinium chloride and from 20-90 degrees C at pH 2.5, and agrees with the known scaling behavior for a multitude of chemically denatured states. The polypeptide is behaving as a statistical coil in the non-interacting, high-temperature limit.Entities:
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Year: 2007 PMID： 17292402 DOI： 10.1016/j.jmb.2007.01.012
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469