Literature DB >> 172887

Partial purification of a glucocorticoid receptor.

D Failla, G M Tomkins, D V Santi.   

Abstract

A simple method for purification of the glucocorticoid receptor from hepatoma tissue culture cells has been developed. The procedure, which requires only about 24 hr, involves biospecific adsorption of the receptor to deoxycorticosterone derivatized agarose, elution with a glucocorticoid, and gel filtration. The receptor-steroid complex is obtained in 35-40% yield and is about 2000-fold purified. It possesses properties similar to those reported in crude extracts, including sedimentation coefficient in glycerol gradients and activation-dependent binding to nuclei.

Entities:  

Mesh：

Substances：

Year:  1975        PMID： 172887      PMCID： PMC433093          DOI： 10.1073/pnas.72.10.3849

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

1.  Acidophilic activation of steroid hormone receptors.

Authors:  E Milgrom; M Atger; E E Baulieu
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

2.  A filter assay for steroid hormone receptors.

Authors:  D V Santi; C H Sibley; E R Perriard; G M Tomkins; J D Baxter
Journal:  Biochemistry       Date:  1973-06-19       Impact factor: 3.162

3.  Affinity chromatography and the purification of estrogen receptors.

Authors:  V Sica; I Parikh; E Nola; G A Puca; P Cuatrecasas
Journal:  J Biol Chem       Date:  1973-09-25       Impact factor: 5.157

4.  Liver cytosol corticosteroid binder II, a hormone receptor.

Authors:  G Litwack; R Filler; S A Rosenfield; N Lichtash; C A Wishman; S Singer
Journal:  J Biol Chem       Date:  1973-11-10       Impact factor: 5.157

5.  Early events in glucocorticoid action. Activation of the steroid receptor and its subsequent specific nuclear binding studied in a cell-free system.

Authors:  S J Higgins; G G Rousseau; J D Baxter; G M Tomkins
Journal:  J Biol Chem       Date:  1973-08-25       Impact factor: 5.157

6.  Relation of steroid structure to enzyme induction in hepatoma tissue culture cells.

Authors:  H H Samuels; G M Tomkins
Journal:  J Mol Biol       Date:  1970-08-28       Impact factor: 5.469

7.  Glucocorticoid receptors: relations between steroid binding and biological effects.

Authors:  G G Rousseau; J D Baxter; G M Tomkins
Journal:  J Mol Biol       Date:  1972-06-14       Impact factor: 5.469

8.  Steroid-induced nuclear binding of glucocorticoid receptors in intact hepatoma cells.

Authors:  G G Rousseau; J D Baxter; S J Higgins; G M Tomkins
Journal:  J Mol Biol       Date:  1973-09-25       Impact factor: 5.469

9.  Specific cytoplasmic glucocorticoid hormone receptors in hepatoma tissue culture cells.

Authors:  J D Baxter; G M Tomkins
Journal:  Proc Natl Acad Sci U S A       Date:  1971-05       Impact factor: 11.205

10.  Use of esters of N-hydroxysuccinimide in the synthesis of N-acylamino acids.

Authors:  Y Lapidot; S Rappoport; Y Wolman
Journal:  J Lipid Res       Date:  1967-03       Impact factor: 5.922
View more
  3 in total

Review 1.  Third-generation Mineralocorticoid Receptor Antagonists: Why Do We Need a Fourth?

Authors:  Elise P Gomez-Sanchez
Journal:  J Cardiovasc Pharmacol       Date:  2016-01       Impact factor: 3.105

2.  Maximizing the purification of the activated glucocorticoid receptor by DNA-cellulose chromatography.

Authors:  H J Eisen; W H Glinsmann
Journal:  Biochem J       Date:  1978-04-01       Impact factor: 3.857

3.  Unique long-acting antiglucocorticoid in whole and broken cell systems.

Authors:  S S Simons; E B Thompson; D F Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.