Literature DB >> 17278879

Effect of blood handling on extracellular Hsp72 concentration after high-intensity exercise in humans.

M Whitham1, M B Fortes.   

Abstract

Heat shock protein 72 (Hsp72) has been detected in the peripheral circulation of humans. Because intracellular Hsp72 binds to aggregated proteins, we hypothesized that postexercise plasma-derived Hsp72 concentrations would be greater than serum-derived Hsp72 because of binding of Hsp72 to aggregated clotting proteins in serum. Postexercise serum, heparin, and ethylenediaminetetraacetic acid (EDTA) samples were collected from 9 recreationally active males and were analyzed for Hsp72 by enzyme-linked immunosorbent assay. In line with our hypothesis, EDTA-treated blood was significantly higher in Hsp72 concentration than all other treatments (P < or = 0.001), whilst heparin plasma (LH) was significantly higher than serum derived on ice (SI) and at room temperature (SR) (P < 0.05; EDTA: 6.46 +/- 0.76, LH: 2.73 +/- 2.26, SI: 0.13 +/- 0.24, SR: 0.20 +/- 0.32 ng/mL). Because previous research has tended to report serum data at the lowest point of the detectable range of the assay, it is recommended that EDTA specimen tubes be used in future investigations.

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Year:  2006        PMID： 17278879      PMCID： PMC1712678          DOI： 10.1379/csc-212.1

Source DB:  PubMed          Journal:  Cell Stress Chaperones        ISSN： 1355-8145            Impact factor:   3.667


  23 in total

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2.  Exercise increases serum Hsp72 in humans.

Authors:  R C Walsh; I Koukoulas; A Garnham; P L Moseley; M Hargreaves; M A Febbraio
Journal:  Cell Stress Chaperones       Date:  2001-10       Impact factor: 3.667

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4.  Exercise intensity and duration affect blood soluble HSP72.

Authors:  E Fehrenbach; A M Niess; K Voelker; H Northoff; F C Mooren
Journal:  Int J Sports Med       Date:  2005-09       Impact factor: 3.118

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Authors:  A Asea; S K Kraeft; E A Kurt-Jones; M A Stevenson; L B Chen; R W Finberg; G C Koo; S K Calderwood
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Authors:  G I Lancaster; K Møller; B Nielsen; N H Secher; M A Febbraio; L Nybo
Journal:  Cell Stress Chaperones       Date:  2004       Impact factor: 3.667
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  7 in total

1.  Commentary to: 'A comparison of two commercially available ELISA methods for the quantification of human plasma heat shock protein 70 during rest and exercise stress' by Lee et al. 2015.

Authors:  Janina Schwarzer; Gabriele Multhoff
Journal:  Cell Stress Chaperones       Date:  2015-08-04       Impact factor: 3.667

2.  The effect of 15 consecutive days of heat-exercise acclimation on heat shock protein 70.

Authors:  Marie E Sandström; Jason C Siegler; Ric J Lovell; Leigh A Madden; Lars McNaughton
Journal:  Cell Stress Chaperones       Date:  2008-02-19       Impact factor: 3.667

3.  The effect of acute hypoxia on heat shock protein 72 expression and oxidative stress in vivo.

Authors:  Lee Taylor; Adrian W Midgley; Bryna Chrismas; Leigh A Madden; Rebecca V Vince; Lars R McNaughton
Journal:  Eur J Appl Physiol       Date:  2010-03-13       Impact factor: 3.078

4.  A comparison of two commercially available ELISA methods for the quantification of human plasma heat shock protein 70 during rest and exercise stress.

Authors:  B J Lee; N M Sukri; H Ogden; C Vine; C D Thake; J E Turner; J L J Bilzon
Journal:  Cell Stress Chaperones       Date:  2015-06-26       Impact factor: 3.667

5.  Heat stress and cardiovascular, hormonal, and heat shock proteins in humans.

Authors:  Masaki Iguchi; Andrew E Littmann; Shuo-Hsiu Chang; Lydia A Wester; Jane S Knipper; Richard K Shields
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Authors:  Flávio de Castro Magalhães; Fabiano Trigueiro Amorim; Renata L Freitas Passos; Michele Atalla Fonseca; Kenya Paula Moreira Oliveira; Milene Rodrigues Malheiros Lima; Juliana Bohen Guimarães; João Batista Ferreira-Júnior; Angelo R P Martini; Nilo R V Lima; Danusa Dias Soares; Edilamar Menezes Oliveira; Luiz Oswaldo Carneiro Rodrigues
Journal:  Cell Stress Chaperones       Date:  2010-04-23       Impact factor: 3.667

Review 7.  Heat shock protein 72 response to exercise in humans.

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Journal:  Sports Med       Date:  2008       Impact factor: 11.136
  7 in total

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