| Literature DB >> 17275939 |
Rodrigo Alonso1, Estibaliz Mateo, Ramón Cisterna.
Abstract
A Real-time PCR assay was developed by using Taqman-MGB probes to screen mutations at codons Ser79 and Asp83 of Streptococcus pneumoniae parC. One hundred and thirty levofloxacin-susceptible and forty-two levofloxacin-resistant clinical strains were assayed. Mutations at codon 79 were found among all the levofloxacin-resistant strains. Mutations at codon 79 or 83 were found in ten levofloxacin-susceptible strains. This procedure is a reliable method for a rapid detection of mutations in the QRDRs of parC gene of S. pneumoniae and could be carried out in a diagnostic laboratory for some high-risk patients or in epidemiological surveys.Entities:
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Year: 2006 PMID： 17275939 DOI： 10.1016/j.mimet.2006.12.016
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363