Makoto Ito1, Masao Ohbayashi, Mitsuru Furukawa, Shigeo Okoyama. 1. Department of Otolaryngology-Head and Neck Surgery, Division of Neuroscience, Laboratory of Neuroanatomy, Center for Biomedical Research and Education, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan. makoto@med.kanazawa-u.ac.jp
Abstract
OBJECTIVE: We investigated free radical scavenging activity and the possible neuroprotective effect of Tokishakuyakusan (TJ-23) on facial motor nucleus (FMN) motoneurons after peripheral axotomy. STUDY DESIGN: In 40 adult rats, the right facial nerve was transected at the level of the stylomastoid foramen. Following axotomy, the effects of TJ-23 on nitric oxide synthase were investigated using NADPH-d histochemistry. FMN motoneurons were counted bilaterally in sections stained with cresyl violet. RESULTS: Rats administered TJ-23 exhibited clear suppression of injury-induced neuronal NADPH-d expression in the ipsilateral FMN when compared to nontreated controls. The number of surviving motoneurons in the ipsilateral FMN was significantly greater among TJ-23-treated rats than nontreated controls on day 56 following axotomy. CONCLUSION: The present study demonstrates the neuroprotective effect of TJ-23 after peripheral facial nerve axotomy. SIGNIFICANCE: Antioxidants may have therapeutic potential in traumatic facial nerve dysfunction resulting from head injury, ear surgery, and parotid gland surgery.
OBJECTIVE: We investigated free radical scavenging activity and the possible neuroprotective effect of Tokishakuyakusan (TJ-23) on facial motor nucleus (FMN) motoneurons after peripheral axotomy. STUDY DESIGN: In 40 adult rats, the right facial nerve was transected at the level of the stylomastoid foramen. Following axotomy, the effects of TJ-23 on nitric oxide synthase were investigated using NADPH-d histochemistry. FMN motoneurons were counted bilaterally in sections stained with cresyl violet. RESULTS:Rats administered TJ-23 exhibited clear suppression of injury-induced neuronal NADPH-d expression in the ipsilateral FMN when compared to nontreated controls. The number of surviving motoneurons in the ipsilateral FMN was significantly greater among TJ-23-treated rats than nontreated controls on day 56 following axotomy. CONCLUSION: The present study demonstrates the neuroprotective effect of TJ-23 after peripheral facial nerve axotomy. SIGNIFICANCE: Antioxidants may have therapeutic potential in traumatic facial nerve dysfunction resulting from head injury, ear surgery, and parotid gland surgery.
Authors: Robin W Lindsay; James T Heaton; Colin Edwards; Christopher Smitson; Kalpesh Vakharia; Tessa A Hadlock Journal: Arch Facial Plast Surg Date: 2010 May-Jun