Literature DB >> 17261511

Simple method for repurification of endotoxins for biological use.

Alina Tirsoaga1, Alexey Novikov, Minou Adib-Conquy, Catherine Werts, Catherine Fitting, Jean-Marc Cavaillon, Martine Caroff.   

Abstract

A method for obtaining highly purified endotoxin (lipopolysaccharide [LPS]) in a few hours by repurification of commercial or laboratory preparations was devised. It avoids the use of phenol, which is not suitable for phenol-soluble lipopolysaccharides nor for some industrial purposes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry analysis confirmed the integrity of the purified LPSs. The purified products did not activate Toll-like receptor 2 (TLR2), nuclear oligomerization domain 1 (NOD1), or NOD2 but did activate TLR4. Applied to different lipopolysaccharides, the method also improved their mass spectra, thus facilitating their structural analysis.

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Year:  2007        PMID： 17261511      PMCID： PMC1828799          DOI： 10.1128/AEM.02452-06

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  30 in total

1.  Direct microextraction and analysis of rough-type lipopolysaccharides by combined thin-layer chromatography and MALDI mass spectrometry.

Authors:  H Therisod; V Labas; M Caroff
Journal:  Anal Chem       Date:  2001-08-15       Impact factor: 6.986

Review 2.  Structure of bacterial lipopolysaccharides.

Authors:  Martine Caroff; Doris Karibian
Journal:  Carbohydr Res       Date:  2003-11-14       Impact factor: 2.104

3.  Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2.

Authors:  Robert Girard; Thierry Pedron; Satoshi Uematsu; Viviane Balloy; Michel Chignard; Shizuo Akira; Richard Chaby
Journal:  J Cell Sci       Date:  2003-01-15       Impact factor: 5.285

4.  Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2.

Authors:  M Hirschfeld; Y Ma; J H Weis; S N Vogel; J J Weis
Journal:  J Immunol       Date:  2000-07-15       Impact factor: 5.422

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  A new method for the extraction of R lipopolysaccharides.

Authors:  C Galanos; O Lüderitz; O Westphal
Journal:  Eur J Biochem       Date:  1969-06

Review 7.  The chemical structure of bacterial endotoxin in relation to bioactivity.

Authors:  E T Rietschel; T Kirikae; F U Schade; A J Ulmer; O Holst; H Brade; G Schmidt; U Mamat; H D Grimmecke; S Kusumoto
Journal:  Immunobiology       Date:  1993-04       Impact factor: 3.144

8.  Improved techniques for the preparation of bacterial lipopolysaccharides.

Authors:  K G Johnson; M B Perry
Journal:  Can J Microbiol       Date:  1976-01       Impact factor: 2.419

9.  Lipid A components from Pseudomonas aeruginosa PAO1 (serotype O5) and mutant strains investigated by electrospray ionization ion-trap mass spectrometry.

Authors:  Gilles Bedoux; Karine Vallée-Réhel; Oliver Kooistra; Ulrich Zähringer; Dominique Haras
Journal:  J Mass Spectrom       Date:  2004-05       Impact factor: 1.982

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Authors:  M Caroff; D R Bundle; M B Perry
Journal:  Eur J Biochem       Date:  1984-02-15
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  16 in total

1.  An important question: Which LPS do you use?

Authors:  Raphael Parusel; Alex Steimle; Anna Lange; Andrea Schäfer; Jan K Maerz; Annika Bender; Julia-Stefanie Frick
Journal:  Virulence       Date:  2017-09-12       Impact factor: 5.882

2.  Substitution of the Bordetella pertussis lipid A phosphate groups with glucosamine is required for robust NF-kappaB activation and release of proinflammatory cytokines in cells expressing human but not murine Toll-like receptor 4-MD-2-CD14.

Authors:  Nico Marr; Adeline M Hajjar; Nita R Shah; Alexey Novikov; Cathy S Yam; Martine Caroff; Rachel C Fernandez
Journal:  Infect Immun       Date:  2010-02-22       Impact factor: 3.441

3.  Lipopolysaccharides belonging to different Salmonella serovars are differentially capable of activating Toll-like receptor 4.

Authors:  Daniela Chessa; Luisella Spiga; Nicola De Riu; Paola Delaconi; Vittorio Mazzarello; Giulia Ganau; Salvatore Rubino
Journal:  Infect Immun       Date:  2014-08-18       Impact factor: 3.441

4.  Structural and biological characteristics of different forms of V. filiformis lipid A: use of MS to highlight structural discrepancies.

Authors:  Aude Breton; Alexey Novikov; Richard Martin; Pierre Tissieres; Martine Caroff
Journal:  J Lipid Res       Date:  2017-01-25       Impact factor: 5.922

5.  Deciphering tissue-induced Klebsiella pneumoniae lipid A structure.

Authors:  Enrique Llobet; Verónica Martínez-Moliner; David Moranta; Käthe M Dahlström; Verónica Regueiro; Anna Tomás; Victoria Cano; Camino Pérez-Gutiérrez; Christian G Frank; Helena Fernández-Carrasco; José Luis Insua; Tiina A Salminen; Junkal Garmendia; José A Bengoechea
Journal:  Proc Natl Acad Sci U S A       Date:  2015-11-02       Impact factor: 11.205

6.  Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response.

Authors:  Catalina March; Verónica Regueiro; Enrique Llobet; David Moranta; Pau Morey; Junkal Garmendia; José A Bengoechea
Journal:  PLoS One       Date:  2010-04-07       Impact factor: 3.240

7.  Structural and biological diversity of lipopolysaccharides from Burkholderia pseudomallei and Burkholderia thailandensis.

Authors:  Vidhya Novem; Guanghou Shui; Dongling Wang; Anne K Bendt; Siew Hoon Sim; Yichun Liu; Tuck Weng Thong; Suppiah Paramalingam Sivalingam; Eng Eong Ooi; Markus R Wenk; Gladys Tan
Journal:  Clin Vaccine Immunol       Date:  2009-08-19

8.  Microwave-assisted sample preparation for rapid and sensitive analysis of H. pylori lipid A applicable to a single colony.

Authors:  Ping Zhou; Vandana Chandan; Xin Liu; Kenneth Chan; Eleonora Altman; Jianjun Li
Journal:  J Lipid Res       Date:  2009-04-28       Impact factor: 5.922

9.  Glucosamine found as a substituent of both phosphate groups in Bordetella lipid A backbones: role of a BvgAS-activated ArnT ortholog.

Authors:  Nico Marr; Alina Tirsoaga; Didier Blanot; Rachel Fernandez; Martine Caroff
Journal:  J Bacteriol       Date:  2008-04-18       Impact factor: 3.490

10.  Minor modifications to the phosphate groups and the C3' acyl chain length of lipid A in two Bordetella pertussis strains, BP338 and 18-323, independently affect Toll-like receptor 4 protein activation.

Authors:  Nita R Shah; Sami Albitar-Nehme; Emma Kim; Nico Marr; Alexey Novikov; Martine Caroff; Rachel C Fernandez
Journal:  J Biol Chem       Date:  2013-03-06       Impact factor: 5.157
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