Literature DB >> 17260182

Detection of rabies virus genes by in-situ polymerase chain reaction.

P Ezhil Praveena1, R Jayakumar, C Balachandran, G Thirumurugan, G Dhinakar Raj, B Murali Manohar.   

Abstract

Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the 'N' gene of rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas.

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Year:  2007        PMID: 17260182     DOI: 10.1007/s11259-007-3492-8

Source DB:  PubMed          Journal:  Vet Res Commun        ISSN: 0165-7380            Impact factor:   2.459


  11 in total

1.  In situ reverse transcription-polymerase chain reaction: a novel technique for detection of rabies virus RNA in murine neuroblastoma cells.

Authors:  R Jayakumar; K G Tirumurugaan; G Ganga; K P Sheela; K Vijayarani; A M Nainar
Journal:  Acta Virol       Date:  2003       Impact factor: 1.162

2.  Detection of rabies virus genomic RNA and mRNA in mouse and human brains by using in situ hybridization.

Authors:  A C Jackson; W H Wunner
Journal:  J Virol       Date:  1991-06       Impact factor: 5.103

3.  Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses.

Authors:  P R Heaton; P Johnstone; L M McElhinney; R Cowley; E O'Sullivan; J E Whitby
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

4.  In situ hybridization, immunohistochemistry, and in situ reverse transcription-polymerase chain reaction for detection of infectious bursal disease virus.

Authors:  X Liu; J J Giambrone; E J Hoerr
Journal:  Avian Dis       Date:  2000 Jan-Mar       Impact factor: 1.577

5.  Polymerase chain reaction in situ: intracellular amplification and detection of HIV-1 proviral DNA and other specific genes.

Authors:  O Bagasra; T Seshamma; R J Pomerantz
Journal:  J Immunol Methods       Date:  1993-01-14       Impact factor: 2.303

6.  Molecular detection of rabies encephalitis and correlation with cytokine expression.

Authors:  Gerard J Nuovo; Dulcelena L Defaria; Juan G Chanona-Vilchi; Yilan Zhang
Journal:  Mod Pathol       Date:  2005-01       Impact factor: 7.842

7.  Effects of post-mortem autolysis on the detection of rabies virus genomic RNA and mRNA in mouse brain by using in situ hybridization.

Authors:  A C Jackson; N E Rintoul
Journal:  Mol Cell Probes       Date:  1992-06       Impact factor: 2.365

8.  In situ detection of PCR-amplified HIV-1 nucleic acids and tumor necrosis factor cDNA in cervical tissues.

Authors:  G J Nuovo; A Forde; P MacConnell; R Fahrenwald
Journal:  Am J Pathol       Date:  1993-07       Impact factor: 4.307

9.  Detection of rabies virus mRNA in mouse brain by using in situ hybridization with digoxigenin-labelled RNA probes.

Authors:  A C Jackson
Journal:  Mol Cell Probes       Date:  1992-04       Impact factor: 2.365

10.  Use of discriminatory probes for strain typing of formalin-fixed, rabies virus-infected tissues by in situ hybridization.

Authors:  Susan A Nadin-Davis; Mary Sheen; Alexander I Wandeler
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

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