Literature DB >> 1513342

Detection of rabies virus mRNA in mouse brain by using in situ hybridization with digoxigenin-labelled RNA probes.

A C Jackson1.   

Abstract

A non-isotopic method of in situ hybridization (ISH) was developed for the detection of rabies virus RNA in paraffin-embedded tissues. Digoxigenin-labelled RNA probes for rabies virus glycoprotein mRNA were used. The method had good sensitivity and low backgrounds, and there was excellent cellular localization of signals. ISH wih digoxigenin-labelled probes was compared with ISH with 3H-labelled probes. This non-isotopic method of ISH is more convenient than the radiolabelled method, and it is quicker because a long autoradiographic exposure is not required.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1513342     DOI： 10.1016/0890-8508(92)90057-5

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  4 in total

1.  Detection of rabies virus genes by in-situ polymerase chain reaction.

Authors:  P Ezhil Praveena; R Jayakumar; C Balachandran; G Thirumurugan; G Dhinakar Raj; B Murali Manohar
Journal:  Vet Res Commun       Date:  2007-01-27       Impact factor: 2.459

2.  Use of discriminatory probes for strain typing of formalin-fixed, rabies virus-infected tissues by in situ hybridization.

Authors:  Susan A Nadin-Davis; Mary Sheen; Alexander I Wandeler
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

3.  Genome-wide identification and expression analysis of SWEET gene family in Litchi chinensis reveal the involvement of LcSWEET2a/3b in early seed development.

Authors:  Hanhan Xie; Dan Wang; Yaqi Qin; Anna Ma; Jiaxin Fu; Yonghua Qin; Guibing Hu; Jietang Zhao
Journal:  BMC Plant Biol       Date:  2019-11-14       Impact factor: 4.215

4.  Detection of rabbit haemorrhagic disease virus (RHDV) by in situ hybridisation with a digoxigenin labelled RNA probe.

Authors:  D Gelmetti; V Grieco; C Rossi; L Capucci; A Lavazza
Journal:  J Virol Methods       Date:  1998-06       Impact factor: 2.014
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.