OBJECTIVE: The kinetics of recovery for innate immune effectors following antiretroviral therapy are unknown. DESIGN AND METHODS: Multiple sequential cryopreserved samples (viremic and ART-suppressed) from 66 patients enrolled in the Women's Interagency HIV Study or Multicenter AIDS Cohort Study cohorts (median follow-up, 700 days) were analyzed to determine natural killer, dendritic and T-cell changes by flow cytometry. Functional parameters were also measured in a subset of samples. Changes over time were analyzed by mixed-effect modeling based on a linear spline with a single knot at 270 days. RESULTS: Following viral suppression, a rapid rise in CD4 and white blood cell counts and a decline in T-cell activation were confirmed. However, natural killer cell subsets increased after 270 days of therapy, with a negative effect by baseline CD4%. CD123+ plasmacytoid but not myeloid dendritic cells showed a trend to increase during the first 270 days with a positive effect of baseline CD4%; plasmacytoid dendritic cell-induced interferon-alpha production significantly increased by end of follow-up. CONCLUSIONS: The kinetics of natural killer and plasmacytoid dendritic cell recovery are markedly different from those of T-cell subsets, indicative of early and delayed benefits of suppressive regimens.
OBJECTIVE: The kinetics of recovery for innate immune effectors following antiretroviral therapy are unknown. DESIGN AND METHODS: Multiple sequential cryopreserved samples (viremic and ART-suppressed) from 66 patients enrolled in the Women's Interagency HIV Study or Multicenter AIDS Cohort Study cohorts (median follow-up, 700 days) were analyzed to determine natural killer, dendritic and T-cell changes by flow cytometry. Functional parameters were also measured in a subset of samples. Changes over time were analyzed by mixed-effect modeling based on a linear spline with a single knot at 270 days. RESULTS: Following viral suppression, a rapid rise in CD4 and white blood cell counts and a decline in T-cell activation were confirmed. However, natural killer cell subsets increased after 270 days of therapy, with a negative effect by baseline CD4%. CD123+ plasmacytoid but not myeloid dendritic cells showed a trend to increase during the first 270 days with a positive effect of baseline CD4%; plasmacytoid dendritic cell-induced interferon-alpha production significantly increased by end of follow-up. CONCLUSIONS: The kinetics of natural killer and plasmacytoid dendritic cell recovery are markedly different from those of T-cell subsets, indicative of early and delayed benefits of suppressive regimens.
Authors: Emmanouil Papasavvas; Jihed Chehimi; Livio Azzoni; Maxwell Pistilli; Brian Thiel; Agnieszka Mackiewicz; Shenoa Creer; Karam Mounzer; Jay R Kostman; Luis J Montaner Journal: AIDS Res Hum Retroviruses Date: 2010-08-18 Impact factor: 2.205
Authors: E Papasavvas; L Azzoni; X Yin; Q Liu; J Joseph; A Mackiewicz; B Ross; K M Lynn; J M Jacobson; K Mounzer; J R Kostman; L J Montaner Journal: J Viral Hepat Date: 2017-05-11 Impact factor: 3.728
Authors: Livio Azzoni; Nigel J Crowther; Cynthia Firnhaber; Andrea S Foulkes; Xiangfan Yin; Deborah Glencross; Robert Gross; Mitch D Kaplan; Emmanouil Papasavvas; Doreen Schulze; Wendy Stevens; Tessa van der Merwe; Rita Waisberg; Ian Sanne; Luis J Montaner Journal: J Int AIDS Soc Date: 2010-09-07 Impact factor: 5.396
Authors: Michelle Y-H Lee; Amit A Upadhyay; Hasse Walum; Chi N Chan; Reem A Dawoud; Christine Grech; Justin L Harper; Kirti A Karunakaran; Sydney A Nelson; Ernestine A Mahar; Kyndal L Goss; Diane G Carnathan; Barbara Cervasi; Kiran Gill; Gregory K Tharp; Elizabeth R Wonderlich; Vijayakumar Velu; Simon M Barratt-Boyes; Mirko Paiardini; Guido Silvestri; Jacob D Estes; Steven E Bosinger Journal: PLoS Pathog Date: 2021-06-28 Impact factor: 6.823