BACKGROUND: An association between plane tree pollen allergy and plant food allergy has been described, but the cross-reacting allergens have not yet been identified. The aim of this study was the identification of homologous non-specific lipid-transfer proteins (nsLTPs) in plane pollen, and to investigate its immunological relationship with the peach LTP, Pru p 3. METHODS: Three different patient groups were recruited in Spain: 22 plane pollen-allergic patients without food allergy (A), 36 plane pollen-allergic patients with peach allergy (B) and 10 peach-allergic patients without plane pollen allergy (C). Proteins from plane pollen extract were fractionated by ion-exchange and reversed-phase chromatography. Further methods applied were N-terminal amino acid sequence analysis, immunoblotting, enzyme allergosorbent test, CAP and basophil histamine release assays. RESULTS: A 10 kDa IgE-reactive protein was purified from plane pollen and identified as nsLTP. Pla a 3 was characterized as a minor allergen (27.3%) in plane pollen-allergic patients without food allergy (A) and as a major allergen in plane pollen-allergic patients with peach allergy (B) showing a prevalence of IgE-reactivity of 63.8%. Group B contained patients sensitized to Pru p 3 without IgE-reactivity to plane-LTP (16.6%). By contrast, Pla a 3 IgE-reactive patients without sensitization to Pru p 3 could be found (16.6%). The sera of patients sensitized to both LTPs (50%), Pla a 3 and Pru p 3, showed different biological activity in histamine release assay: depending on individual patient's sera tested, Pla a 3 showed a similar, a stronger or a weaker allergenic potency in comparison with Pru p 3. CONCLUSIONS: Plane LTP is a major allergen in plane pollen-allergic patients with peach allergy recruited in the Mediterranean area. The results of histamine release tests and different IgE-binding profiles pointed towards the existence of species-specific IgE epitopes. Likewise, no general conclusion on the sensitizer could be made.
BACKGROUND: An association between plane tree pollen allergy and plant food allergy has been described, but the cross-reacting allergens have not yet been identified. The aim of this study was the identification of homologous non-specific lipid-transfer proteins (nsLTPs) in plane pollen, and to investigate its immunological relationship with the peach LTP, Pru p 3. METHODS: Three different patient groups were recruited in Spain: 22 plane pollen-allergicpatients without food allergy (A), 36 plane pollen-allergicpatients with peachallergy (B) and 10 peach-allergicpatients without plane pollen allergy (C). Proteins from plane pollen extract were fractionated by ion-exchange and reversed-phase chromatography. Further methods applied were N-terminal amino acid sequence analysis, immunoblotting, enzyme allergosorbent test, CAP and basophil histamine release assays. RESULTS: A 10 kDa IgE-reactive protein was purified from plane pollen and identified as nsLTP. Pla a 3 was characterized as a minor allergen (27.3%) in plane pollen-allergicpatients without food allergy (A) and as a major allergen in plane pollen-allergicpatients with peachallergy (B) showing a prevalence of IgE-reactivity of 63.8%. Group B contained patients sensitized to Pru p 3 without IgE-reactivity to plane-LTP (16.6%). By contrast, Pla a 3 IgE-reactive patients without sensitization to Pru p 3 could be found (16.6%). The sera of patients sensitized to both LTPs (50%), Pla a 3 and Pru p 3, showed different biological activity in histamine release assay: depending on individual patient's sera tested, Pla a 3 showed a similar, a stronger or a weaker allergenic potency in comparison with Pru p 3. CONCLUSIONS: Plane LTP is a major allergen in plane pollen-allergicpatients with peachallergy recruited in the Mediterranean area. The results of histamine release tests and different IgE-binding profiles pointed towards the existence of species-specific IgE epitopes. Likewise, no general conclusion on the sensitizer could be made.
Authors: Isabel J Skypala; Ricardo Asero; Domingo Barber; Lorenzo Cecchi; Arazeli Diaz Perales; Karin Hoffmann-Sommergruber; Elide A Pastorello; Ines Swoboda; Joan Bartra; Didier G Ebo; Margaretha A Faber; Montserrat Fernández-Rivas; Francesca Gomez; Anastasios P Konstantinopoulos; Olga Luengo; Ronald van Ree; Enrico Scala; Stephen J Till Journal: Clin Transl Allergy Date: 2021-05-18 Impact factor: 5.871
Authors: Arantxa Palacín; Luis A Rivas; Cristina Gómez-Casado; Jacobo Aguirre; Leticia Tordesillas; Joan Bartra; Carlos Blanco; Teresa Carrillo; Javier Cuesta-Herranz; José A Cumplido Bonny; Enrique Flores; Mar G García-Alvarez-Eire; Ignacio García-Nuñez; Francisco J Fernández; Pedro Gamboa; Rosa Muñoz; Rosa Sánchez-Monge; Maria Torres; Susana Varela Losada; Mayte Villalba; Francisco Vega; Victor Parro; Miguel Blanca; Gabriel Salcedo; Araceli Díaz-Perales Journal: PLoS One Date: 2012-09-07 Impact factor: 3.240
Authors: Arantxa Palacín; Cristina Gómez-Casado; Luis A Rivas; Jacobo Aguirre; Leticia Tordesillas; Joan Bartra; Carlos Blanco; Teresa Carrillo; Javier Cuesta-Herranz; Consolación de Frutos; Genoveva García Alvarez-Eire; Francisco J Fernández; Pedro Gamboa; Rosa Muñoz; Rosa Sánchez-Monge; Sofía Sirvent; María J Torres; Susana Varela-Losada; Rosalía Rodríguez; Victor Parro; Miguel Blanca; Gabriel Salcedo; Araceli Díaz-Perales Journal: PLoS One Date: 2012-12-14 Impact factor: 3.240
Authors: Maria Livia Bernardi; Ivana Giangrieco; Laura Camardella; Rosetta Ferrara; Paola Palazzo; Maria Rosaria Panico; Roberta Crescenzo; Vito Carratore; Danila Zennaro; Marina Liso; Mario Santoro; Sara Zuzzi; Maurizio Tamburrini; Maria Antonietta Ciardiello; Adriano Mari Journal: PLoS One Date: 2011-11-17 Impact factor: 3.240