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Literature DB >> 1724597

Antikeratin 14 monoclonal antibody staining in psoriasis and seborrhoeic keratosis: immunofluorescence and two colour FACS studies.

H Wongwaisayawan¹, T Yoshiike, Y Aikawa, R A Briggaman, H Ogawa.

Abstract

A monoclonal antibody (ES3A) was raised against a mouse graft-versus-host reaction (GVHR) model. This antibody was against basal cell cytoplasm and reacted with an acidic (pI 6.2) 50 kDa keratin of human epidermis. However, ES3A reacted with several lower layers of epidermal cells in psoriasis and seborrhoeic keratosis. Acanthotic seborrhoeic keratosis showed varying patterns even in a single lesion. If combined with FACS analysis, ES3A-positive cells could be quantified. Normal skin showed 28%, while psoriasis and seborrhoeic keratosis showed 44% and 51%, respectively. ES3A-positive compartments of the acanthotic type of seborrhoeic keratosis were larger than those of the hyperkeratotic type. ES3A may be suitable for quantification of germinative or proliferative cells.

Entities: Disease Species

Mesh：

Substances：

Year: 1991 PMID： 1724597 DOI： 10.1007/bf00371824

Source DB: PubMed Journal: Arch Dermatol Res ISSN： 0340-3696 Impact factor: 3.017

21 in total

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Authors: S H Dairkee; B M Ljung; H Smith; A Hackett
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5. Monoclonal antikeratin antibody: production, characterization, and immunohistochemical application.

Authors: H Eto; K Hashimoto; H Kobayashi; M Matsumoto; T Kanzaki; A H Mehregan; R A Weiss
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6. Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization.

Authors: B Hentzer; T Kobayasi
Journal: Acta Derm Venereol Date: 1976 Impact factor: 4.437

7. Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes.

Authors: R A Weiss; R Eichner; T T Sun
Journal: J Cell Biol Date: 1984-04 Impact factor: 10.539

2. An immunohistochemical and histochemical study of cytokeratin, involucrin and transglutaminase in seborrhoeic keratosis.

Authors: D Broekaert; I M Leigh; E B Lane; G N Van Muijen; F C Ramaekers; J De Bersaques; P Coucke
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2 in total

Antikeratin 14 monoclonal antibody staining in psoriasis and seborrhoeic keratosis: immunofluorescence and two colour FACS studies.

1. Quantitative single cell analysis and sorting.

2. Characterization of breast carcinomas by two monoclonal antibodies distinguishing myoepithelial from luminal epithelial cells.

3. Immunolocalization of a human basal epithelium specific keratin in benign and malignant breast disease.

4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

5. Monoclonal antikeratin antibody: production, characterization, and immunohistochemical application.

6. Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization.

7. Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes.

8. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.

9. Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.

10. Chronic allogeneic disease. I. Development of glomerulonephritis.

1. Is an epitope on keratin 17 a major target for autoreactive T lymphocytes in psoriasis?

2. An immunohistochemical and histochemical study of cytokeratin, involucrin and transglutaminase in seborrhoeic keratosis.