S Scholl1, M Hanke, K Höffken, H G Sayer. 1. Department of Internal Medicine II (Oncology and Hematology), Friedrich Schiller University, Erlanger Allee 101, 07740 Jena, Germany. sebastian.scholl@med.uni-jena.de
Abstract
PURPOSE: The study investigated in detail neutrophil functions shortly after allogeneic peripheral blood stem cell transplantation (PBSCT). METHODS: Different functions of neutrophils in 14 patients who received allogeneic PBSCT were investigated. The migratory capacity as well as the ability to induce oxidative burst following stimulation with either Phorbol-12-myristate-13-acetate (PMA), the chemotactic peptide N-formyl-Met-Leu-Phe (f-MLP) or opsonized Escherichia coli was analysed after engraftment (between day +30 and +40) and compared with the results obtained from healthy volunteers. RESULTS: There are no differences in terms of the migratory capacity (P = 0.17), as well as regarding the oxidative burst after incubation with PMA (P = 0.08) or f-MLP (P = 0.06), compared with healthy men. In contrast, the capacity of neutrophils to induce oxidative burst following stimulation with E. coli is highly impaired (P = 0.0001) in patients shortly after engraftment. CONCLUSION: The recovery of neutrophils after allogeneic PBSCT is not only influenced by the varying time of engraftment, but also represents a process that differs in distinct biological functions compared to normal granulopoieses.
PURPOSE: The study investigated in detail neutrophil functions shortly after allogeneic peripheral blood stem cell transplantation (PBSCT). METHODS: Different functions of neutrophils in 14 patients who received allogeneic PBSCT were investigated. The migratory capacity as well as the ability to induce oxidative burst following stimulation with either Phorbol-12-myristate-13-acetate (PMA), the chemotactic peptide N-formyl-Met-Leu-Phe (f-MLP) or opsonized Escherichia coli was analysed after engraftment (between day +30 and +40) and compared with the results obtained from healthy volunteers. RESULTS: There are no differences in terms of the migratory capacity (P = 0.17), as well as regarding the oxidative burst after incubation with PMA (P = 0.08) or f-MLP (P = 0.06), compared with healthy men. In contrast, the capacity of neutrophils to induce oxidative burst following stimulation with E. coli is highly impaired (P = 0.0001) in patients shortly after engraftment. CONCLUSION: The recovery of neutrophils after allogeneic PBSCT is not only influenced by the varying time of engraftment, but also represents a process that differs in distinct biological functions compared to normal granulopoieses.
Authors: Maraike Rommeley; Baerbel Spies-Weisshart; Kristina Schilling; Andreas Hochhaus; Herbert G Sayer; Sebastian Scholl Journal: J Cancer Res Clin Oncol Date: 2011-06-29 Impact factor: 4.553
Authors: Racquel Domingo-Gonzalez; Giovanny J Martínez-Colón; Alana J Smith; Carolyne K Smith; Megan N Ballinger; Meng Xia; Susan Murray; Mariana J Kaplan; Gregory A Yanik; Bethany B Moore Journal: Am J Respir Crit Care Med Date: 2016-01-15 Impact factor: 21.405
Authors: C R Y Cruz; S Lam; P J Hanley; A S Bear; C Langston; A J Cohen; H Liu; C A Martinez; R A Krance; H E Heslop; C M Rooney; I C Hanson; C M Bollard Journal: Clin Exp Immunol Date: 2013-10 Impact factor: 4.330
Authors: Anna Thunström Salzer; Maria J Niemiec; Ava Hosseinzadeh; Marios Stylianou; Fredrik Åström; Marc Röhm; Clas Ahlm; Anders Wahlin; David Ermert; Constantin F Urban Journal: Front Immunol Date: 2018-09-11 Impact factor: 7.561