PURPOSE: The aim of the study was to investigate the recovery of the innate immune system within the first 100 days after allogeneic peripheral blood stem cell transplantation (PBSCT) and to elucidate a potential correlation with such important events as severe infectious complications or graft-versus-host disease (GvHD). METHODS: In 30 consecutive patients who underwent allogeneic PBSCT, absolute numbers of neutrophils and monocytes were determined and different functional analyses performed at different time points (day +30, +60 and +90, respectively). The capacity to phagocyte Escherichia coli (E. coli) as well as the induction of oxidative burst after incubation with different stimuli (Phorbol-12-myristate-13-acetate; PMA, the chemotactic peptide N-formyl-Met-Leu-Phe; f-MLP or opsonized E. coli) were analysed after engraftment. RESULTS: There was a rapid reconstitution concerning the capability of both neutrophils and monocytes to phagocyte E. coli without a significant increase between day +30 and +90. In contrast, a twofold increase of monocyte oxidative burst after incubation with PMA at day +90 was observed (P = 0.017). Furthermore, the ability of neutrophils to induce oxidative burst after ingestion with E. coli was impaired on day +30 with a significant functional reconstitution on day +60 (P = 0.01). The oxidative burst activity following incubation with f-MLP did not show significant changes after stem cell engraftment. Analysis of numeric reconstitution of CD14+CD16+ monocytes demonstrated a potential correlation with a decreased incidence of chronic GvHD. CONCLUSION: The functional recovery of neutrophils and monocytes in the early period after allogeneic PBSCT differs not only concerning phagocytosis and oxidative burst but also with respect to the stimulus and the cell population that was analysed for oxidative burst activity. The subset of CD16+CD14+ monocytes might be a predictor for a reduced risk of chronic GvHD.
PURPOSE: The aim of the study was to investigate the recovery of the innate immune system within the first 100 days after allogeneic peripheral blood stem cell transplantation (PBSCT) and to elucidate a potential correlation with such important events as severe infectious complications or graft-versus-host disease (GvHD). METHODS: In 30 consecutive patients who underwent allogeneic PBSCT, absolute numbers of neutrophils and monocytes were determined and different functional analyses performed at different time points (day +30, +60 and +90, respectively). The capacity to phagocyte Escherichia coli (E. coli) as well as the induction of oxidative burst after incubation with different stimuli (Phorbol-12-myristate-13-acetate; PMA, the chemotactic peptide N-formyl-Met-Leu-Phe; f-MLP or opsonized E. coli) were analysed after engraftment. RESULTS: There was a rapid reconstitution concerning the capability of both neutrophils and monocytes to phagocyte E. coli without a significant increase between day +30 and +90. In contrast, a twofold increase of monocyte oxidative burst after incubation with PMA at day +90 was observed (P = 0.017). Furthermore, the ability of neutrophils to induce oxidative burst after ingestion with E. coli was impaired on day +30 with a significant functional reconstitution on day +60 (P = 0.01). The oxidative burst activity following incubation with f-MLP did not show significant changes after stem cell engraftment. Analysis of numeric reconstitution of CD14+CD16+ monocytes demonstrated a potential correlation with a decreased incidence of chronic GvHD. CONCLUSION: The functional recovery of neutrophils and monocytes in the early period after allogeneic PBSCT differs not only concerning phagocytosis and oxidative burst but also with respect to the stimulus and the cell population that was analysed for oxidative burst activity. The subset of CD16+CD14+ monocytes might be a predictor for a reduced risk of chronic GvHD.
Authors: M N Saleh; S J Goldman; A F LoBuglio; A C Beall; H Sabio; M C McCord; L Minasian; R K Alpaugh; L M Weiner; D H Munn Journal: Blood Date: 1995-05-15 Impact factor: 22.113
Authors: Lucie M Turcotte; Qing Cao; Sarah A Cooley; Julie Curtsinger; Shernan G Holtan; Xianghua Luo; Ashely Yingst; Daniel J Weisdorf; Bruce R Blazar; Jeffrey S Miller; John E Wagner; Michael R Verneris Journal: Biol Blood Marrow Transplant Date: 2019-01-06 Impact factor: 5.742
Authors: E Mimiola; O Marini; O Perbellini; A Micheletti; W Vermi; S Lonardi; C Costantini; E Meneghelli; A Andreini; C Bonetto; A Vassanelli; M Cantini; E Zoratti; D Massi; A Zamo'; A Leso; G Quaresmini; F Benedetti; G Pizzolo; M A Cassatella; C Tecchio Journal: Clin Exp Immunol Date: 2014-10 Impact factor: 4.330
Authors: Birgit Sawitzki; Claudio Brunstein; Christian Meisel; Julia Schumann; Katrin Vogt; Christine Appelt; Julie M Curtsinger; Michael R Verneris; Jeffrey S Miller; John E Wagner; Bruce R Blazar Journal: Biol Blood Marrow Transplant Date: 2013-11-01 Impact factor: 5.742
Authors: Lauren Stern; Helen McGuire; Selmir Avdic; Simone Rizzetto; Barbara Fazekas de St Groth; Fabio Luciani; Barry Slobedman; Emily Blyth Journal: Front Immunol Date: 2018-07-26 Impact factor: 7.561
Authors: Harini D de Silva; Rosemary A Ffrench; Maya Korem; Eva Orlowski; David J Curtis; Andrew Spencer; Sharon Avery; Sushrut Patil; Catherine Orla Morrissey Journal: Clin Transl Immunology Date: 2018-10-05
Authors: Sejal Morjaria; Allen W Zhang; Sohn Kim; Jonathan U Peled; Simone Becattini; Eric R Littmann; Eric G Pamer; Michael C Abt; Miguel-Angel Perales Journal: Clin Hematol Int Date: 2020-11-23