Literature DB >> 172435

Elevation of adenosine 3',5'-cyclic monophosphate in established mamalian cell strains by Hypaque (sodium diatrizoate).

J C Patrick, S Rengachary, G Melnykovych.   

Abstract

Hypaque (sodium diatrizoate), when added to cells growing in culture (two HeLa S3 strains, rat glioblastoma C6 and mouse lymphoma L5178Y), increased the intracellular level of adenosine 3',5'-cyclic monophosphate. A transient elevation of adenosine 3',5'-cyclic monophosphate was also observed when L5178Y cells were subjected to a procedure recommended for separation of lymphocytes from peripheral blood. The effect of Hypaque did not appear to be related to the increase in osmolality of the medium.

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Year:  1975        PMID： 172435     DOI： 10.1007/bf02616378

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  10 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Plasma membrane cyclic adenosine 3':5'-monophosphate phosphodiesterase of cultured cells and its modification after trypsin treatment of intact cells.

Authors:  T Russell; I Pastan
Journal:  J Biol Chem       Date:  1973-08-25       Impact factor: 5.157

3.  Interrelationship between adenylate cyclase activity, adenosine 3':5' cyclic monophosphate phosphodiesterase activity, adenosine 3':5' cyclic monophosphate levels, and growth of cells in culture.

Authors:  W B Anderson; T R Russell; R A Carchman; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

4.  The use of Metrizamide as a gradient medium for isopycnic separation of rat liver cells.

Authors:  A C Munthe-Kaas; P O Seglen
Journal:  FEBS Lett       Date:  1974-08-01       Impact factor: 4.124

5.  The uses of metrizamide in the fractionation of nuclei from brain and liver tissue by zonal centrifugation.

Authors:  A P Mathias; C V Wynter
Journal:  FEBS Lett       Date:  1973-06-15       Impact factor: 4.124

6.  Ficoll-isopaque gradients for the determination of density distributions of human blood lymphocytes and other reticulo-endothelial cells.

Authors:  J A Loos; D Roos
Journal:  Exp Cell Res       Date:  1974-06       Impact factor: 3.905

7.  Effects of prednisolone on the distribution and activity of two phosphoesterases in membrane preparations from HeLa cells.

Authors:  M E Costlow; G Melnykrovych
Journal:  J Cell Physiol       Date:  1972-10       Impact factor: 6.384

8.  Basophil leucocyte separation from human peripheral blood: a technique for their isolation in high purity and high yield.

Authors:  R P Day
Journal:  Clin Allergy       Date:  1972-09

9.  Serum-dependent depression of alkaline phosphatase in HeLa cells.

Authors:  F Herz; E Kaplan; D A Sevdalian
Journal:  J Cell Physiol       Date:  1969-10       Impact factor: 6.384

10.  A protein binding assay for adenosine 3':5'-cyclic monophosphate.

Authors:  A G Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1970-09       Impact factor: 11.205
  10 in total
  1 in total

1.  Induction of alkaline phosphatase activity in HeLa cells by sodium butyrate.

Authors:  W Wharton; C A Hart; B Goz
Journal:  In Vitro       Date:  1979-11
  1 in total

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