Literature DB >> 1723622

A common disinfectant used in condom processing inhibits endonuclease digestion of sperm DNA.

S Fadda1, S Pelotti, G Pappalardo.   

Abstract

DNA recovered from a condom found at a crime scene appeared undigestible with restriction enzymes, preventing characterization by Southern blot and polymorphic probe hybridization. Several chemical substances used in the processing and treatment of condoms were tested for inhibitory action of restriction enzymes. In particular dibenzalkonium chloride appeared to promote enzyme inhibition at very low concentrations. The effectiveness of treatments to restore cleavage of sample DNA in the presence of such contaminants is discussed.

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Year:  1991        PMID： 1723622     DOI： 10.1007/bf01369586

Source DB:  PubMed          Journal:  Int J Legal Med        ISSN： 0937-9827            Impact factor:   2.686


  9 in total

1.  A specificity enhancer for polymerase chain reaction.

Authors:  T Hung; K Mak; K Fong
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

2.  The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains.

Authors:  M Prinz; G Berghaus
Journal:  Z Rechtsmed       Date:  1990

3.  Analysis of the human alpha-globin gene cluster reveals a highly informative genetic locus.

Authors:  D R Higgs; J S Wainscoat; J Flint; A V Hill; S L Thein; R D Nicholls; H Teal; H Ayyub; T E Peto; A G Falusi
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

4.  Application of deoxyribonucleic acid (DNA) polymorphisms to the analysis of DNA recovered from sperm.

Authors:  A Giusti; M Baird; S Pasquale; I Balazs; J Glassberg
Journal:  J Forensic Sci       Date:  1986-04       Impact factor: 1.832

5.  Genomic sequencing.

Authors:  G M Church; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

6.  "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1984-02       Impact factor: 3.365

7.  Rapid transfer of DNA from agarose gels to nylon membranes.

Authors:  K C Reed; D A Mann
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

8.  Forensic application of DNA 'fingerprints'.

Authors:  P Gill; A J Jeffreys; D J Werrett
Journal:  Nature       Date:  1985 Dec 12-18       Impact factor: 49.962

9.  The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing.

Authors:  G Del Sal; G Manfioletti; C Schneider
Journal:  Biotechniques       Date:  1989-05       Impact factor: 1.993
  9 in total

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