Literature DB >> 1720557

M-15: high-affinity chimeric peptide that blocks the neuronal actions of galanin in the hippocampus, locus coeruleus, and spinal cord.

T Bartfai1, K Bedecs, T Land, U Langel, R Bertorelli, P Girotti, S Consolo, X J Xu, Z Wiesenfeld-Hallin, S Nilsson.   

Abstract

The 20-amino acid peptide M-15 binds with high affinity (IC50 approximately 0.1 nM) to 125I-labeled galanin (125I-GAL) binding sites in membranes from the ventral hippocampus, midbrain, and rat spinal cord. Receptor autoradiographic studies show that M-15 can displace 125I-GAL from all labeled sites. M-15 acts as a reversible high-affinity antagonist in blocking the inhibitory effects of GAL on the evoked release of acetylcholine in vivo in the hippocampus and on the GAL-induced hyperpolarization of locus coeruleus neurons in slices. M-15 also blocks the facilitatory effects of GAL on the spinal flexor reflex. Thus, the chimeric peptide M-15 [GAL-(1-13)-substance P-(5-11)amide] represents the first antagonist to the neuronal actions of GAL.

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Year:  1991        PMID： 1720557      PMCID： PMC53052          DOI： 10.1073/pnas.88.23.10961

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

1.  Evidence for norepinephrine-mediated collateral inhibition of locus coeruleus neurons.

Authors:  G K Aghajanian; J M Cedarbaum; R Y Wang
Journal:  Brain Res       Date:  1977-11-18       Impact factor: 3.252

2.  N-terminal galanin-(1-16) fragment is an agonist at the hippocampal galanin receptor.

Authors:  G Fisone; M Berthold; K Bedecs; A Undén; T Bartfai; R Bertorelli; S Consolo; J Crawley; B Martin; S Nilsson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

3.  Galanin blocks the slow cholinergic EPSP in CA1 pyramidal neurons from ventral hippocampus.

Authors:  P Dutar; Y Lamour; R A Nicoll
Journal:  Eur J Pharmacol       Date:  1989-05-19       Impact factor: 4.432

4.  Evidence for self- and neighbor-mediated postactivation inhibition of locus coeruleus neurons.

Authors:  M Ennis; G Aston-Jones
Journal:  Brain Res       Date:  1986-05-28       Impact factor: 3.252

5.  Membrane properties of rat locus coeruleus neurones.

Authors:  J T Williams; R A North; S A Shefner; S Nishi; T M Egan
Journal:  Neuroscience       Date:  1984-09       Impact factor: 3.590

6.  Fine structure of rat locus coeruleus.

Authors:  P M Groves; C J Wilson
Journal:  J Comp Neurol       Date:  1980-10-15       Impact factor: 3.215

7.  The effects of intrathecal galanin and C-fiber stimulation on the flexor reflex in the rat.

Authors:  Z Wiesenfeld-Hallin; M J Villar; T Hökfelt
Journal:  Brain Res       Date:  1989-05-08       Impact factor: 3.252

8.  A new method for receptor autoradiography: [3H]opioid receptors in rat brain.

Authors:  W S Young; M J Kuhar
Journal:  Brain Res       Date:  1979-12-28       Impact factor: 3.252

9.  On the Role of Galanin, Substance P and Other Neuropeptides in Primary Sensory Neurons of the Rat: Studies on Spinal Reflex Excitability and Peripheral Axotomy.

Authors:  X.-J. Xu; Z. Wiesenfeld-Hallin; M. J. Villar; J. Fahrenkrug; T. Hökfelt
Journal:  Eur J Neurosci       Date:  1990       Impact factor: 3.386

10.  Coexistence of galanin-like immunoreactivity with catecholamines, 5-hydroxytryptamine, GABA and neuropeptides in the rat CNS.

Authors:  T Melander; T Hökfelt; A Rökaeus; A C Cuello; W H Oertel; A Verhofstad; M Goldstein
Journal:  J Neurosci       Date:  1986-12       Impact factor: 6.167
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  24 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  2010-08-16       Impact factor: 11.205

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Authors:  V I Lyudyno; I N Abdurasulova; V M Klimenko
Journal:  Neurosci Behav Physiol       Date:  2008-01

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Authors:  G A Kinney; P J Emmerson; R J Miller
Journal:  J Neurosci       Date:  1998-05-15       Impact factor: 6.167

4.  The Concise Guide to PHARMACOLOGY 2013/14: G protein-coupled receptors.

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5.  Galanin Activates G Protein Gated Inwardly Rectifying Potassium Channels and Suppresses Kisspeptin-10 Activation of GnRH Neurons.

Authors:  Stephanie Constantin; Susan Wray
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6.  Characterization of a high-affinity galanin receptor in the rat anterior pituitary: absence of biological effect and reduced membrane binding of the antagonist M15 differentiate it from the brain/gut receptor.

Authors:  D Wynick; D M Smith; M Ghatei; K Akinsanya; R Bhogal; P Purkiss; P Byfield; N Yanaihara; S R Bloom
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205

7.  Galanin modulation of seizures and seizure modulation of hippocampal galanin in animal models of status epilepticus.

Authors:  A M Mazarati; H Liu; U Soomets; R Sankar; D Shin; H Katsumori; U Langel; C G Wasterlain
Journal:  J Neurosci       Date:  1998-12-01       Impact factor: 6.167

8.  New high affinity peptide antagonists to the spinal galanin receptor.

Authors:  X J Xu; Z Wiesenfeld-Hallin; U Langel; K Bedecs; T Bartfai
Journal:  Br J Pharmacol       Date:  1995-10       Impact factor: 8.739

9.  Mechanism of galanin's inhibitory action on pancreatic enzyme secretion: modulation of cholinergic transmission--studies in vivo and in vitro.

Authors:  K H Herzig; G Brunke; I Schön; M Schäffer; U R Fölsch
Journal:  Gut       Date:  1993-11       Impact factor: 23.059

10.  Galanin antagonist effects on cardiac vagal inhibitory actions of sympathetic stimulation in anaesthetized cats and dogs.

Authors:  L G Ulman; M Moriarty; E K Potter; D I McCloskey
Journal:  J Physiol       Date:  1993-05       Impact factor: 5.182
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