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Literature DB >> 17196647

Identification and characterization of a novel (S)-ketoprofen-specific esterase.

Sangyoung Yoon¹, SeungBum Kim, Yeonwoo Ryu, T Doohun Kim.

Abstract

A new (S)-ketoprofen specific esterase (EST-Y29) was identified from a metagenome library from environmental samples, which showed homologies with class C-beta lactamase, penicillin binding protein, and other lipases/esterases. In order to investigate the biochemical and biophysical properties, the recombinant protein was overexpressed, purified to homogeneity, and characterized. This EST-Y29 has high catalytic activity against p-nitrophenyl esters of short fatty acids (C(2) and C(4)) and alpha-naphthyl acetate with activation energy of 30.4 kJ/mol. We have further characterized EST-Y29 using high performance liquid chromatography (HPLC), circular dichroism (CD), dynamic light scattering (DLS) and size exclusion chromatography (SEC).

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Year: 2006 PMID： 17196647 DOI： 10.1016/j.ijbiomac.2006.11.010

Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953

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Cited

7 in total

1. Purification, crystallization and preliminary crystallographic analysis of Est-Y29: a novel oligomeric beta-lactamase.

Authors: Seungbum Kim; Sangbum Joo; Sangyoung Yoon; Sungsoo Kim; Jongkook Moon; Yeonwoo Ryu; Kyeong Kyu Kim; T Doohun Kim
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2009-02-26

2. Characterization, crystallization and preliminary X-ray diffraction analysis of an (S)-specific esterase (pfEstA) from Pseudomonas fluorescens KCTC 1767: enantioselectivity for potential industrial applications.

Authors: Seulgi Kim; Tri Duc Ngo; Kyeong Kyu Kim; T Doohun Kim
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2012-10-30

3. Identification, crystallization and preliminary X-ray diffraction analysis of esterase A from Caulobacter crescentus CB15, a family VIII lipolytic enzyme.

Authors: Bum Han Ryu; Tri Duc Ngo; Eunjin Jang; Sungsoo Kim; Hansol Ju; Kyeong Kyu Kim; T Doohun Kim
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2012-04-21

4. Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase.

Authors: SeungBum Kim; Sangbum Joo; Hyun C Yoon; Yeonwoo Ryu; Kyeong Kyu Kim; T Doohun Kim
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2007-06-11

Identification and characterization of a novel (S)-ketoprofen-specific esterase.

1. Purification, crystallization and preliminary crystallographic analysis of Est-Y29: a novel oligomeric beta-lactamase.

2. Characterization, crystallization and preliminary X-ray diffraction analysis of an (S)-specific esterase (pfEstA) from Pseudomonas fluorescens KCTC 1767: enantioselectivity for potential industrial applications.

3. Identification, crystallization and preliminary X-ray diffraction analysis of esterase A from Caulobacter crescentus CB15, a family VIII lipolytic enzyme.

4. Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase.

Review 5. Estimating the success of enzyme bioprospecting through metagenomics: current status and future trends.

6. Molecular Characterization of a Novel Family VIII Esterase with β-Lactamase Activity (PsEstA) from Paenibacillus sp.

7. Discovery and Design of Family VIII Carboxylesterases as Highly Efficient Acyltransferases.