Literature DB >> 1719367

Characterisation of saporin genes: in vitro expression and ribosome inactivation.

A P Fordham-Skelton1, P N Taylor, M R Hartley, R R Croy.   

Abstract

A. Saponaria (soapwort) genomic library was screened with a PCR-derived saporin-specific gene probe. The nucleotide sequences of three saporin genomic clones were determined. One of the clones contained a full-length saporin coding sequence whilst the other two were truncated. A hybrid full-length saporin coding sequence was constructed using the two truncated clones. An SP6 promoter sequence and in-frame initiation codon was added to each of the coding sequences using PCR. In vitro translation of saporin coding sequence transcripts in rabbit reticulocyte lysates resulted in the specific depurination of 28S RNA. This indicated that the saporin sequences encoded functional polypeptides with RNA N-glycosidase activity.

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Year:  1991        PMID： 1719367     DOI： 10.1007/bf00267470

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  38 in total

1.  Purification and partial characterization of the antiviral protein from Phytolacca americana which inhibits eukaryotic protein synthesis.

Authors:  J D Irvin
Journal:  Arch Biochem Biophys       Date:  1975-08       Impact factor: 4.013

2.  Nucleotide sequence of cDNA coding for saporin-6, a type-1 ribosome-inactivating protein from Saponaria officinalis.

Authors:  L Benatti; M B Saccardo; M Dani; G Nitti; M Sassano; R Lorenzetti; D A Lappi; M Soria
Journal:  Eur J Biochem       Date:  1989-08-01

3.  Modification of ribosomal RNA by ribosome-inactivating proteins from plants.

Authors:  F Stirpe; S Bailey; S P Miller; J W Bodley
Journal:  Nucleic Acids Res       Date:  1988-02-25       Impact factor: 16.971

4.  Synthesis of saporin gene probes from partial protein sequence data: use of inosine-oligonucleotides, genomic DNA and the polymerase chain reaction.

Authors:  A P Fordham-Skelton; A Yarwood; R R Croy
Journal:  Mol Gen Genet       Date:  1990-03

5.  Primary amino acid sequence of alpha-trichosanthin and molecular models for abrin A-chain and alpha-trichosanthin.

Authors:  E J Collins; J D Robertus; M LoPresti; K L Stone; K R Williams; P Wu; K Hwang; M Piatak
Journal:  J Biol Chem       Date:  1990-05-25       Impact factor: 5.157

6.  Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N-glycosidase activity of the toxins.

Authors:  Y Endo; K Tsurugi; T Yutsudo; Y Takeda; T Ogasawara; K Igarashi
Journal:  Eur J Biochem       Date:  1988-01-15

7.  The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.

Authors:  U E Loening
Journal:  Biochem J       Date:  1969-06       Impact factor: 3.857

Review 8.  Ribosome-inactivating proteins up to date.

Authors:  F Stirpe; L Barbieri
Journal:  FEBS Lett       Date:  1986-01-20       Impact factor: 4.124

9.  Lambda replacement vectors carrying polylinker sequences.

Authors:  A M Frischauf; H Lehrach; A Poustka; N Murray
Journal:  J Mol Biol       Date:  1983-11-15       Impact factor: 5.469

10.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205
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  4 in total

1.  Characterization of a saporin isoform with lower ribosome-inhibiting activity.

Authors:  M S Fabbrini; E Rappocciolo; D Carpani; M Solinas; B Valsasina; U Breme; U Cavallaro; A Nykjaer; E Rovida; G Legname; M R Soria
Journal:  Biochem J       Date:  1997-03-15       Impact factor: 3.857

2.  Comparative induction of 28S ribosomal RNA cleavage by ricin and the trichothecenes deoxynivalenol and T-2 toxin in the macrophage.

Authors:  Maoxiang Li; James J Pestka
Journal:  Toxicol Sci       Date:  2008-06-04       Impact factor: 4.849

3.  Maize ribosome-inactivating protein (b-32). Homologs in related species, effects on maize ribosomes, and modulation of activity by pro-peptide deletions.

Authors:  T D Hey; M Hartley; T A Walsh
Journal:  Plant Physiol       Date:  1995-04       Impact factor: 8.340

Review 4.  Pokeweed antiviral protein, a ribosome inactivating protein: activity, inhibition and prospects.

Authors:  Artem V Domashevskiy; Dixie J Goss
Journal:  Toxins (Basel)       Date:  2015-01-28       Impact factor: 4.546
  4 in total

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