Generation and use of anti-phosphotyrosine antibodies for immunoblotting.

The techniques of detecting phosphotyrosine-containing proteins by AIA and purifying these proteins by affinity chromatography with anti-phosphotyrosine monoclonal antibodies are both reliable and consistent. Although not every tyrosine-phosphorylated protein can be detected and purified by using these techniques, a majority can. No doubt future studies will employ these approaches both for analyzing the specific role that tyrosine protein phosphorylation plays in regulating cell division and for investigating the properties of heretofore uncharacterized proteins that contain phosphotyrosine.