Literature DB >> 17192851

A flexible protein linker improves the function of epitope-tagged proteins in Saccharomyces cerevisiae.

Michelle Sabourin1, Creighton T Tuzon, Timothy S Fisher, Virginia A Zakian.   

Abstract

Epitope tagging permits the detection of proteins when protein-specific antibodies are not available. However, the epitope tag can reduce the function of the tagged protein. Here we describe a cassette that can be used to introduce an eight amino acid flexible linker between multiple Myc epitopes and the open reading frame of a given gene. We show that inserting the linker improves the in vivo ability of the telomerase subunits Est2p and Est1p to maintain telomere length. The methods used here are generally applicable to improve the function of tagged proteins in both Saccharomyces cerevisiae and Schizosaccharomyces pombe. Copyright (c) 2007 John Wiley & Sons, Ltd.

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Year:  2007        PMID： 17192851      PMCID： PMC3518027          DOI： 10.1002/yea.1431

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  19 in total

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  30 in total

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