| Literature DB >> 17188680 |
Tracy Saveria1, Peter Kessler, Bryan C Jensen, Marilyn Parsons.
Abstract
The glycosomes of trypanosomatids are essential organelles that are evolutionarily related to peroxisomes of other eukaryotes. The peroxisomal RING proteins-PEX2, PEX10 and PEX12-comprise a network of integral membrane proteins that function in the matrix protein import cycle. Here, we describe PEX10 and PEX12 in Trypanosoma brucei, Leishmania major, and Trypanosoma cruzi. We expressed GFP fusions of each T. brucei coding region in procyclic form T. brucei, where they localized to glycosomes and behaved as integral membrane proteins. Despite the weak transmembrane predictions for TbPEX12, protease protection assays demonstrated that both the N and C termini are cytosolic, similar to mammalian PEX12. GFP fusions of T. cruzi PEX10 and L. major PEX12 also localized to glycosomes in T. brucei indicating that glycosomal membrane protein targeting is conserved across trypanosomatids.Entities:
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Year: 2006 PMID： 17188680 PMCID： PMC1976121 DOI： 10.1016/j.exppara.2006.11.004
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011