Literature DB >> 1716622

Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli.

K Muraiso1, M Hijikata, N Kato, K Shimotohno, N Okazaki, S Ohkoshi, M Uura, S Kaneko, K Kobayashi, M Omata.   

Abstract

Enzyme-linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli-expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.

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Year:  1991        PMID: 1716622      PMCID: PMC5918576          DOI: 10.1111/j.1349-7006.1991.tb01914.x

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN: 0910-5050


hepatitis C virus enzyme‐linked immunosorbent assay reverse transcription followed by polymerase chain reaction
  8 in total

1.  Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome.

Authors:  Q L Choo; G Kuo; A J Weiner; L R Overby; D W Bradley; M Houghton
Journal:  Science       Date:  1989-04-21       Impact factor: 47.728

2.  Non-specificity of anti-HCV test for seroepidemiological analysis.

Authors:  D C Wong; A R Diwan; L Rosen; J L Gerin; R G Johnson; A Polito; R H Purcell
Journal:  Lancet       Date:  1990-09-22       Impact factor: 79.321

3.  Treatment of chronic hepatitis C with recombinant interferon alfa. A multicenter randomized, controlled trial.

Authors:  G L Davis; L A Balart; E R Schiff; K Lindsay; H C Bodenheimer; R P Perrillo; W Carey; I M Jacobson; J Payne; J L Dienstag
Journal:  N Engl J Med       Date:  1989-11-30       Impact factor: 91.245

4.  Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.

Authors:  N Kato; M Hijikata; Y Ootsuyama; M Nakagawa; S Ohkoshi; T Sugimura; K Shimotohno
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

5.  A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus.

Authors:  K Muraiso; M Hijikata; S Ohkoshi; M J Cho; M Kikuchi; N Kato; K Shimotohno
Journal:  Biochem Biophys Res Commun       Date:  1990-10-30       Impact factor: 3.575

6.  An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis.

Authors:  G Kuo; Q L Choo; H J Alter; G L Gitnick; A G Redeker; R H Purcell; T Miyamura; J L Dienstag; M J Alter; C E Stevens
Journal:  Science       Date:  1989-04-21       Impact factor: 47.728

7.  Hepatitis C virus antibodies in chronic active hepatitis: pathogenetic factor or false-positive result?

Authors:  I G McFarlane; H M Smith; P J Johnson; G P Bray; D Vergani; R Williams
Journal:  Lancet       Date:  1990-03-31       Impact factor: 79.321

8.  Detection of hepatitis C virus RNA in sera and liver tissues of non-A, non-B hepatitis patients using the polymerase chain reaction.

Authors:  S Ohkoshi; N Kato; T Kinoshita; M Hijikata; Y Ohtsuyama; N Okazaki; H Ohkura; S Hirohashi; A Honma; T Ozaki
Journal:  Jpn J Cancer Res       Date:  1990-09
  8 in total
  3 in total

1.  Synthetic gene for the hepatitis C virus nucleocapsid protein.

Authors:  Y E Khudyakov; H A Fields; M O Favorov; N S Khudyakova; M T Bonafonte; B Holloway
Journal:  Nucleic Acids Res       Date:  1993-06-11       Impact factor: 16.971

2.  Antigenic regions within the hepatitis C virus envelope 1 and non-structural proteins: identification of an IgG3-restricted recognition site with the envelope 1 protein.

Authors:  M Sällberg; U Rudén; B Wahren; L O Magnius
Journal:  Clin Exp Immunol       Date:  1993-03       Impact factor: 4.330

3.  Serodiagnostic assay of hepatitis C virus infection using viral proteins expressed in Escherichia coli.

Authors:  S Mori; S Ohkoshi; M Hijikata; N Kato; K Shimotohno
Journal:  Jpn J Cancer Res       Date:  1992-03
  3 in total

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