Literature DB >> 1700704

A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus.

K Muraiso1, M Hijikata, S Ohkoshi, M J Cho, M Kikuchi, N Kato, K Shimotohno.   

Abstract

A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.

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Year:  1990        PMID： 1700704     DOI： 10.1016/0006-291x(90)90702-o

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  13 in total

1.  Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis.

Authors:  M Hijikata; N Kato; Y Ootsuyama; M Nakagawa; K Shimotohno
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

2.  Identification of four epitopes in hepatitis C virus core protein.

Authors:  P Ferroni; G Mascolo; M Zaninetti; D Colzani; F Pregliasco; M Pirisi; F Barbone; V Gasparini
Journal:  J Clin Microbiol       Date:  1993-06       Impact factor: 5.948

3.  Synthetic gene for the hepatitis C virus nucleocapsid protein.

Authors:  Y E Khudyakov; H A Fields; M O Favorov; N S Khudyakova; M T Bonafonte; B Holloway
Journal:  Nucleic Acids Res       Date:  1993-06-11       Impact factor: 16.971

4.  Humoral immune response to hypervariable region 1 of the putative envelope glycoprotein (gp70) of hepatitis C virus.

Authors:  N Kato; H Sekiya; Y Ootsuyama; T Nakazawa; M Hijikata; S Ohkoshi; K Shimotohno
Journal:  J Virol       Date:  1993-07       Impact factor: 5.103

5.  Expression of hepatitis C virus core protein as a fusion protein with maltose binding protein. Detection of anti-hepatitis C core antibody by western blot.

Authors:  O Yokosuka; Y Ito; J Sakuma; F Imazeki; M Ohto; M Omata
Journal:  Dig Dis Sci       Date:  1993-04       Impact factor: 3.199

6.  Serodiagnosis of hepatitis C virus (HCV) infection with an HCV core protein molecularly expressed by a recombinant baculovirus.

Authors:  J Chiba; H Ohba; Y Matsuura; Y Watanabe; T Katayama; S Kikuchi; I Saito; T Miyamura
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

7.  Genetic drift in hypervariable region 1 of the viral genome in persistent hepatitis C virus infection.

Authors:  N Kato; Y Ootsuyama; H Sekiya; S Ohkoshi; T Nakazawa; M Hijikata; K Shimotohno
Journal:  J Virol       Date:  1994-08       Impact factor: 5.103

8.  Serological reactivity and viral genotypes in hepatitis C virus infection.

Authors:  F Maggi; M L Vatteroni; M Pistello; C M Avio; N Cecconi; F Panicucci; M Bendinelli
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

9.  Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli.

Authors:  K Muraiso; M Hijikata; N Kato; K Shimotohno; N Okazaki; S Ohkoshi; M Uura; S Kaneko; K Kobayashi; M Omata
Journal:  Jpn J Cancer Res       Date:  1991-08

10.  Replication of hepatitis C virus in cultured non-neoplastic human hepatocytes.

Authors:  N Kato; M Ikeda; T Mizutani; K Sugiyama; M Noguchi; S Hirohashi; K Shimotohno
Journal:  Jpn J Cancer Res       Date:  1996-08
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