Chemiluminescence determination of sodium new houttuyfonate in pharmaceutical preparations based on tween 80-rhodamine B system.

Strong chemiluminescence (CL) emission was observed when sodium new houttuyfonate (SNH) was mixed with Tween 80 in sulfuric acid medium in the presence of rhodamine B. Base on this phenomenon, a sensitive flow injection-CL method for the determination of SNH was developed. Under the optimum conditions, the CL emission is linearly with SNH concentration in the range 8.0-4000 ng mL(-1), with a detection limit of 2.7 ng mL(-1) (3sigma). As a preliminary application, the proposed method was successfully applied to the determination of SNH in pharmaceutical preparations. The possible CL mechanism was also discussed in this paper.