Effect of electrode potential on electrode-reducing microbiota.

The benthic microbial fuel cell (BMFC) generates power by coupling oxidation of fuels naturally residing in marine sediments with reduction of oxygen in overlying waters. A central feature of BMFCs is spontaneous colonization of the anode by mineral-reducing microorganisms indigenous to marine sediments that catalyze the power-generating anodic reactions. Described here is a preliminary investigation of how the anode potential affects this feature. Different oxidative potentials were applied to a set of anodes under conditions known to promote anode enrichment of acetate oxidizing/mineral reducing microorganisms. In-situ analysis of current, acetate consumption, and reducing ability of the anode colonies suggest thatthe microorganisms conserve a significant portion (as much as 95%) of potential energy liberated from oxidation of acetate and reduction of the anode for their own metabolic benefit. The implication of this result with respect to BMFCs, and other MFCs utilizing electrode-reducing microbial catalysts, is that although the microorganisms enable long-term stability of such fuel cells, they may significantly impact efficiency of power output per equivalent of fuel consumed.