Literature DB >> 17142904

Overexpression, purification, crystallization and preliminary X-ray analysis of uracil N-glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor.

Prem Singh1, Ramappa K Talawar, P D V Krishna, Umesh Varshney, M Vijayan.   

Abstract

Uracil N-glycosylase is an enzyme which initiates the pathway of uracil-excision repair of DNA. The enzyme from Mycobacterium tuberculosis was co-expressed with a proteinaceous inhibitor from Bacillus subtilis phage and was crystallized in monoclinic space group C2, with unit-cell parameters a = 201.14, b = 64.27, c = 203.68 A, beta = 109.7 degrees. X-ray data from the crystal have been collected for structure analysis.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 17142904      PMCID： PMC2225355          DOI： 10.1107/S1744309106045805

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  36 in total

1.  Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase.

Authors:  C D Putnam; M J Shroyer; A J Lundquist; C D Mol; A S Arvai; D W Mosbaugh; J A Tainer
Journal:  J Mol Biol       Date:  1999-03-26       Impact factor: 5.469

2.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

3.  Uracil-directed ligand tethering: an efficient strategy for uracil DNA glycosylase (UNG) inhibitor development.

Authors:  Yu Lin Jiang; Daniel J Krosky; Lauren Seiple; James T Stivers
Journal:  J Am Chem Soc       Date:  2005-12-14       Impact factor: 15.419

4.  Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication.

Authors:  H Nilsen; I Rosewell; P Robins; C F Skjelbred; S Andersen; G Slupphaug; G Daly; H E Krokan; T Lindahl; D E Barnes
Journal:  Mol Cell       Date:  2000-06       Impact factor: 17.970

5.  Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-DNA N-glycosylase (UNG) from Atlantic cod (Gadus morhua).

Authors:  Elin Moe; Ingar Leiros; Ellen Kristin Riise; Magne Olufsen; Olav Lanes; Arneo Smalås; Nils Peder Willassen
Journal:  J Mol Biol       Date:  2004-11-05       Impact factor: 5.469

6.  Genetic requirements for mycobacterial survival during infection.

Authors:  Christopher M Sassetti; Eric J Rubin
Journal:  Proc Natl Acad Sci U S A       Date:  2003-10-20       Impact factor: 11.205

7.  Partial purification and characterization of a uracil-DNA glycosylase from wheat germ.

Authors:  P Blaisdell; H Warner
Journal:  J Biol Chem       Date:  1983-02-10       Impact factor: 5.157

8.  Herpes simplex virus type 1 uracil-DNA glycosylase: isolation and selective inhibition by novel uracil derivatives.

Authors:  F Focher; A Verri; S Spadari; R Manservigi; J Gambino; G E Wright
Journal:  Biochem J       Date:  1993-06-15       Impact factor: 3.857

9.  DNA deaminating ability and genotoxicity of nitric oxide and its progenitors.

Authors:  D A Wink; K S Kasprzak; C M Maragos; R K Elespuru; M Misra; T M Dunams; T A Cebula; W H Koch; A W Andrews; J S Allen
Journal:  Science       Date:  1991-11-15       Impact factor: 47.728

10.  Sequence analysis, expression, and conservation of Escherichia coli uracil DNA glycosylase and its gene (ung).

Authors:  U Varshney; T Hutcheon; J H van de Sande
Journal:  J Biol Chem       Date:  1988-06-05       Impact factor: 5.157
View more
  3 in total

1.  Crystallization and preliminary X-ray studies of the C-terminal domain of Mycobacterium tuberculosis LexA.

Authors:  Anu V Chandran; J Rajan Prabu; G P Manjunath; K Neelakanteshwar Patil; K Muniyappa; M Vijayan
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-08-28

2.  Structure of uracil-DNA glycosylase from Mycobacterium tuberculosis: insights into interactions with ligands.

Authors:  Prem Singh Kaushal; Ramappa K Talawar; Umesh Varshney; M Vijayan
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-07-27

3.  Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening.

Authors:  Avani Mehta; Prateek Raj; Sandeep Sundriyal; Balasubramanian Gopal; Umesh Varshney
Journal:  Biochem Biophys Rep       Date:  2021-02-15
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.