Literature DB >> 17142903

Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli.

Yu Hirano1, Md Motarab Hossain, Kazuki Takeda, Hajime Tokuda, Kunio Miki.   

Abstract

The outer membrane lipoprotein NlpE functions in stress response by activating the Cpx signal transduction pathway. The nonlipidated Cys1Ala mutant of NlpE with a C-terminal His tag from Escherichia coli was constructed, overexpressed and purified. Crystals of NlpE were grown in two distinct forms by the sitting-drop vapour-diffusion method at 298 K. The tetragonal crystals diffracted to 2.8 A resolution and belong to space group P4(3)2(1)2. The monoclinic crystals diffracted to 3.0 A resolution and belong to space group C2. Initial phases were obtained from a tetragonal crystal of selenomethionylated protein by the MAD method.

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Year:  2006        PMID： 17142903      PMCID： PMC2225377          DOI： 10.1107/S1744309106045313

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  32 in total

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Authors:  T Yakushi; K Masuda; S Narita; S Matsuyama; H Tokuda
Journal:  Nat Cell Biol       Date:  2000-04       Impact factor: 28.824

2.  Substructure solution with SHELXD.

Authors:  Thomas R Schneider; George M Sheldrick
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-09-28

3.  A single amino acid determinant of the membrane localization of lipoproteins in E. coli.

Authors:  K Yamaguchi; F Yu; M Inouye
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4.  Elucidation of the function of lipoprotein-sorting signals that determine membrane localization.

Authors:  Kazuhiro Masuda; Shin-ichi Matsuyama; Hajime Tokuda
Journal:  Proc Natl Acad Sci U S A       Date:  2002-05-28       Impact factor: 11.205

5.  Lipoprotein sorting signals evaluated as the LolA-dependent release of lipoproteins from the cytoplasmic membrane of Escherichia coli.

Authors:  M Terada; T Kuroda; S I Matsuyama; H Tokuda
Journal:  J Biol Chem       Date:  2001-10-09       Impact factor: 5.157

6.  The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP.

Authors:  P N Danese; W B Snyder; C L Cosma; L J Davis; T J Silhavy
Journal:  Genes Dev       Date:  1995-02-15       Impact factor: 11.361

7.  Tethering of CpxP to the inner membrane prevents spheroplast induction of the cpx envelope stress response.

Authors:  T L Raivio; M W Laird; J C Joly; T J Silhavy
Journal:  Mol Microbiol       Date:  2000-09       Impact factor: 3.501

8.  Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli.

Authors:  Kaneyoshi Yamamoto; Akira Ishihama
Journal:  Biosci Biotechnol Biochem       Date:  2006-07       Impact factor: 2.043

9.  Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway.

Authors:  Karen Otto; Thomas J Silhavy
Journal:  Proc Natl Acad Sci U S A       Date:  2002-02-05       Impact factor: 11.205

10.  A third envelope stress signal transduction pathway in Escherichia coli.

Authors:  Robert G Raffa; Tracy L Raivio
Journal:  Mol Microbiol       Date:  2002-09       Impact factor: 3.501
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  1 in total

1.  Treponema pallidum Lipoprotein TP0435 Expressed in Borrelia burgdorferi Produces Multiple Surface/Periplasmic Isoforms and mediates Adherence.

Authors:  Kamfai Chan; Thayer Nasereddin; Laura Alter; Arturo Centurion-Lara; Lorenzo Giacani; Nikhat Parveen
Journal:  Sci Rep       Date:  2016-05-10       Impact factor: 4.379
  1 in total

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