| Literature DB >> 17140782 |
J C A Tanaka1, C C da Silva, I C P Ferreira, G M C Machado, L L Leon, A J B de Oliveira.
Abstract
The present study was designated to evaluate the antileishmanial activity of acid and basic fractions that were obtained after acid-basic extraction, from ethanolic 70% crude extract and pure compounds from the stem bark of Aspidosperma ramiflorum. The basic alkaloidal fraction presented a good activity against the extracellular form (promastigotes) of Leishmania (L.) amazonensis (LD(50) value<47 microg/ml). Based on these findings, the basic fraction was fractionated on silica gel column chromatography in a bioassay-guided fractionation affording individual purified ramiflorines A and B. Both ramiflorines A and B showed significant activity against Leishmania (L.) amazonensis (LD(50) values of 16.3+/-1.6 microg/ml and 4.9+/-0.9 microg/ml, respectively). Our results are promising, showing that these compounds are biologically active against Gram-positive bacteria.Entities:
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Year: 2006 PMID： 17140782 DOI： 10.1016/j.phymed.2006.09.002
Source DB: PubMed Journal: Phytomedicine ISSN： 0944-7113 Impact factor: 5.340