Literature DB >> 1712997

UNME/K1: an IgG2a monoclonal antibody specific to cytokeratin of human urinary bladder squamous cell carcinoma.

H el-Mohamady1, M T Basta, M N Seddek, H Helmy, E al-Hilaly, A M Attallah, M A Ghoneim.   

Abstract

The main distinctive feature of carcinoma in schistosomal bladder is keratinized squamous cell carcinoma. Keratins/cytokeratins constitute a multigeneic family of structurally related polypeptide markers for the malignant state of epithelial cells. A monoclonal antibody (UNME/K1) regognizing keratins associated with squamous cell carcinoma of the human urinary bladder was generated at the Urology and Nephrology Center, Mansoura, Egypt (UNME), by fusion of spleenocytes from a BALB/c mouse immunized with a keratin extract (K1) of human squamous cell carcinoma and P3X63Ag8/U1 syngeneic myeloma cells. UNME/K1 was purified by a protein-A affinity column and was of the IgG2a type, as determined by immunoelectrophoresis and gel diffusion techniques. When tested against keratins of different types of urinary bladder tumors using enzym linked immunosorbent assay (ELISA), UNME/K1 reacted only with the high molecular weight keratin of squamous cell carcinoma and showed selectivity towards specific histopathological grades of tumors.

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Year:  1991        PMID： 1712997     DOI： 10.1007/bf00368194

Source DB:  PubMed          Journal:  Urol Res        ISSN： 0300-5623


  18 in total

1.  Variation and frequency of cytokeratin polypeptide patterns in human squamous non-keratinizing epithelium.

Authors:  G A Wild; D Mischke
Journal:  Exp Cell Res       Date:  1986-01       Impact factor: 3.905

2.  NCL-5D3: a new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue.

Authors:  B Angus; J Purvis; D Stock; B R Westley; A C Samson; E G Routledge; F H Carpenter; C H Horne
Journal:  J Pathol       Date:  1987-12       Impact factor: 7.996

Review 3.  Intermediate filaments: a chemically heterogeneous, developmentally regulated class of proteins.

Authors:  E Lazarides
Journal:  Annu Rev Biochem       Date:  1982       Impact factor: 23.643

Review 4.  Production of monoclonal antibodies: strategy and tactics.

Authors:  S F de StGroth; D Scheidegger
Journal:  J Immunol Methods       Date:  1980       Impact factor: 2.303

5.  Studies of the IgM and IgA contamination obtained by eluting IgG from protein A-Sepharose column with pH steps.

Authors:  M A Vidal; F P Conde
Journal:  J Immunol Methods       Date:  1980       Impact factor: 2.303

6.  Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13.

Authors:  G N van Muijen; D J Ruiter; W W Franke; T Achtstätter; W H Haasnoot; M Ponec; S O Warnaar
Journal:  Exp Cell Res       Date:  1986-01       Impact factor: 3.905

7.  Liver tumors distinguished by immunofluorescence microscopy with antibodies to proteins of intermediate-sized filaments.

Authors:  P Bannasch; H Zerban; E Schmid; W W Franke
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

8.  Mitosis and intermediate-sized filaments in developing skeletal muscle.

Authors:  H Ishikawa; R Bischoff; H Holtzer
Journal:  J Cell Biol       Date:  1968-09       Impact factor: 10.539

9.  Cytokeratin No. 9, an epidermal type I keratin characteristic of a special program of keratinocyte differentiation displaying body site specificity.

Authors:  A C Knapp; W W Franke; H Heid; M Hatzfeld; J L Jorcano; R Moll
Journal:  J Cell Biol       Date:  1986-08       Impact factor: 10.539

10.  Detection of a cytokeratin determinant common to diverse epithelial cells by a broadly cross-reacting monoclonal antibody.

Authors:  O Gigi; B Geiger; Z Eshhar; R Moll; E Schmid; S Winter; D L Schiller; W W Franke
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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