Interpretation of electron density with stereographic roadmap projections.

The program RIVEM (Radial Interpretation of Viral Electron density Maps) was developed to project density radially onto a sphere that is then presented as a stereographic diagram. This permits features resulting from an asymmetric reconstruction to be projected and positioned onto an icosahedral virus surface. The features that constitute the viral surface can also be simultaneously represented in terms of atoms, amino acid residues, potential charge distribution, and surface topology. The procedure can also be adapted for the investigation of various molecular interactions.