| Literature DB >> 1710949 |
Abstract
The human breast cancer cell lines T47D and MCF-7, respond to the mitogenic action of exogenously added PDGF with a 2-3-fold increase in cell number. The maximal response was obtained at a concentration of 1.25 half maximal units/ml (125 ng/ml). PDGF-AA was even more effective than PDGF-AB while PDGF-BB was without effect. The growth-enhancing activity of PDGF was completely abolished by Fab fragments of anti PDGF. Within 7 min of addition of PDGF to cultures of T47D cells, specific phosphorylation of a 65-kDa protein was observed. T47D cells contain specific receptors for PDGF with approximately 4-7 x 10(4) sites (kDa of 3-4 x 10(-10) M) per cell.Entities:
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Year: 1991 PMID： 1710949 DOI： 10.1016/0304-3835(91)90036-h
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679