BACKGROUND: Gastric cancer often metastasizes to the peritoneal cavity in which tumor cells are exposed to hypoxia without systemic circulation. Hypoxia-inducible factor-1 (HIF-1) and its target gene, vascular endothelial growth factor (VEGF), may play a role in the development of peritoneal metastases. MATERIALS AND METHODS: The mRNA levels of HIF-1 alpha and VEGF were examined in 21 normal gastric mucosa, 158 primary tumors and 18 peritoneal metastases by quantitative RT-PCR. RESULTS: HIF-1 alpha and VEGF were significantly up-regulated in the peritoneal metastases compared with those of the normal mucosa and the primary tumors. A positive correlation between HIF-1 alpha and VEGF was observed in the peritoneal dissemination. CONCLUSION: These results suggest that HIF-1 alpha and its target gene, VEGF, were up-regulated in the intraperitoneal tumors but not in the primary cancers of the stomach. Different microenvironments may influence the expressions of these genes.
BACKGROUND:Gastric cancer often metastasizes to the peritoneal cavity in which tumor cells are exposed to hypoxia without systemic circulation. Hypoxia-inducible factor-1 (HIF-1) and its target gene, vascular endothelial growth factor (VEGF), may play a role in the development of peritoneal metastases. MATERIALS AND METHODS: The mRNA levels of HIF-1 alpha and VEGF were examined in 21 normal gastric mucosa, 158 primary tumors and 18 peritoneal metastases by quantitative RT-PCR. RESULTS:HIF-1 alpha and VEGF were significantly up-regulated in the peritoneal metastases compared with those of the normal mucosa and the primary tumors. A positive correlation between HIF-1 alpha and VEGF was observed in the peritoneal dissemination. CONCLUSION: These results suggest that HIF-1 alpha and its target gene, VEGF, were up-regulated in the intraperitoneal tumors but not in the primary cancers of the stomach. Different microenvironments may influence the expressions of these genes.
Authors: N R Sluiter; E M V de Cuba; R Kwakman; W J H J Meijerink; P M Delis-van Diemen; V M H Coupé; J A M Beliën; G A Meijer; I H J T de Hingh; E A te Velde Journal: Clin Exp Metastasis Date: 2016-02-12 Impact factor: 5.150
Authors: E M V de Cuba; I H J T de Hingh; N R Sluiter; R Kwakman; V M H Coupé; J A M Beliën; V J Verwaal; W J H J Meijerink; P M Delis-van Diemen; H J Bonjer; G A Meijer; E A Te Velde Journal: Ann Surg Oncol Date: 2016-01-04 Impact factor: 5.344