Literature DB >> 17084816

A PCR-based method for detection and quantification of small RNAs.

Seungil Ro1, Chanjae Park, Jingling Jin, Kenton M Sanders, Wei Yan.   

Abstract

Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method.

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Year:  2006        PMID： 17084816      PMCID： PMC1934510          DOI： 10.1016/j.bbrc.2006.10.105

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  37 in total

1.  Identification of many microRNAs that copurify with polyribosomes in mammalian neurons.

Authors:  John Kim; Anna Krichevsky; Yonatan Grad; Gabriel D Hayes; Kenneth S Kosik; George M Church; Gary Ruvkun
Journal:  Proc Natl Acad Sci U S A       Date:  2003-12-22       Impact factor: 11.205

2.  Evaluation of 15 polymerases and phosphorothioate primer modification for detection of UV-induced C:G to T:A mutations by allele-specific PCR.

Authors:  James M Gale; Gregory B Tafoya
Journal:  Photochem Photobiol       Date:  2004-05       Impact factor: 3.421

3.  A high-throughput method to monitor the expression of microRNA precursors.

Authors:  Thomas D Schmittgen; Jinmai Jiang; Qian Liu; Liuqing Yang
Journal:  Nucleic Acids Res       Date:  2004-02-25       Impact factor: 16.971

4.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

5.  Detection of single DNA base differences by competitive oligonucleotide priming.

Authors:  R A Gibbs; P N Nguyen; C T Caskey
Journal:  Nucleic Acids Res       Date:  1989-04-11       Impact factor: 16.971

6.  Altered expression profiles of microRNAs during TPA-induced differentiation of HL-60 cells.

Authors:  Katsumi Kasashima; Yoshikazu Nakamura; Tomoko Kozu
Journal:  Biochem Biophys Res Commun       Date:  2004-09-17       Impact factor: 3.575

7.  The nuclear RNase III Drosha initiates microRNA processing.

Authors:  Yoontae Lee; Chiyoung Ahn; Jinju Han; Hyounjeong Choi; Jaekwang Kim; Jeongbin Yim; Junho Lee; Patrick Provost; Olof Rådmark; Sunyoung Kim; V Narry Kim
Journal:  Nature       Date:  2003-09-25       Impact factor: 49.962

8.  Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival.

Authors:  Junichi Takamizawa; Hiroyuki Konishi; Kiyoshi Yanagisawa; Shuta Tomida; Hirotaka Osada; Hideki Endoh; Tomoko Harano; Yasushi Yatabe; Masato Nagino; Yuji Nimura; Tetsuya Mitsudomi; Takashi Takahashi
Journal:  Cancer Res       Date:  2004-06-01       Impact factor: 12.701

9.  Human embryonic stem cells express a unique set of microRNAs.

Authors:  Mi-Ra Suh; Yoontae Lee; Jung Yeon Kim; Soo-Kyoung Kim; Sung-Hwan Moon; Ji Yeon Lee; Kwang-Yul Cha; Hyung Min Chung; Hyun Soo Yoon; Shin Yong Moon; V Narry Kim; Kye-Seong Kim
Journal:  Dev Biol       Date:  2004-06-15       Impact factor: 3.582

10.  Microarray analysis of microRNA expression in the developing mammalian brain.

Authors:  Eric A Miska; Ezequiel Alvarez-Saavedra; Matthew Townsend; Akira Yoshii; Nenad Sestan; Pasko Rakic; Martha Constantine-Paton; H Robert Horvitz
Journal:  Genome Biol       Date:  2004-08-31       Impact factor: 13.583
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  62 in total

1.  MicroRNA regulated defense responses in Triticum aestivum L. during Puccinia graminis f.sp. tritici infection.

Authors:  Om Prakash Gupta; Vipin Permar; Vikas Koundal; Uday Dhari Singh; Shelly Praveen
Journal:  Mol Biol Rep       Date:  2011-06-02       Impact factor: 2.316

2.  Discovery and characterization of novel microRNAs during endothelial differentiation of human embryonic stem cells.

Authors:  Jung Ki Yoo; Jumi Kim; Seong-Jun Choi; Hye Min Noh; Young Do Kwon; Hanna Yoo; Hyo Seon Yi; Hyung Min Chung; Jin Kyeoung Kim
Journal:  Stem Cells Dev       Date:  2012-02-07       Impact factor: 3.272

3.  miR-ID: a novel, circularization-based platform for detection of microRNAs.

Authors:  Pavan Kumar; Brian H Johnston; Sergei A Kazakov
Journal:  RNA       Date:  2010-12-17       Impact factor: 4.942

4.  A microRNA encoded by Autographa californica nucleopolyhedrovirus regulates expression of viral gene ODV-E25.

Authors:  Mengxiao Zhu; Jinwen Wang; Riqiang Deng; Peiwen Xiong; Hai Liang; Xunzhang Wang
Journal:  J Virol       Date:  2013-09-11       Impact factor: 5.103

5.  Cloning and expression profiling of small RNAs expressed in the mouse ovary.

Authors:  Seungil Ro; Rui Song; Chanjae Park; Huili Zheng; Kenton M Sanders; Wei Yan
Journal:  RNA       Date:  2007-10-19       Impact factor: 4.942

6.  Cloning and expression profiling of testis-expressed microRNAs.

Authors:  Seungil Ro; Chanjae Park; Kenton M Sanders; John R McCarrey; Wei Yan
Journal:  Dev Biol       Date:  2007-09-18       Impact factor: 3.582

7.  Cloning and expression profiling of testis-expressed piRNA-like RNAs.

Authors:  Seungil Ro; Chanjae Park; Rui Song; Dan Nguyen; Jingling Jin; Kenton M Sanders; John R McCarrey; Wei Yan
Journal:  RNA       Date:  2007-08-13       Impact factor: 4.942

8.  Sertoli cell Dicer is essential for spermatogenesis in mice.

Authors:  Marilena D Papaioannou; Jean-Luc Pitetti; Seungil Ro; Chanjae Park; Florence Aubry; Olivier Schaad; Charles E Vejnar; Francoise Kühne; Patrick Descombes; Evgeny M Zdobnov; Michael T McManus; Florian Guillou; Brian D Harfe; Wei Yan; Bernard Jégou; Serge Nef
Journal:  Dev Biol       Date:  2008-11-28       Impact factor: 3.582

9.  MicroRNA-29b regulates ethanol-induced neuronal apoptosis in the developing cerebellum through SP1/RAX/PKR cascade.

Authors:  Yuanlin Qi; Mingfang Zhang; Hui Li; Jacqueline A Frank; Lu Dai; Huijuan Liu; Gang Chen
Journal:  J Biol Chem       Date:  2014-02-19       Impact factor: 5.157

Review 10.  Detection of miRNAs with a nanopore single-molecule counter.

Authors:  Li-Qun Gu; Meni Wanunu; Michael X Wang; Larry McReynolds; Yong Wang
Journal:  Expert Rev Mol Diagn       Date:  2012-07       Impact factor: 5.225
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