Literature DB >> 17084472

Evaluation of eight RNA isolation methods for transcriptional analysis in Campylobacter jejuni.

Vongsavanh Phongsisay1, Viraj N Perera, Benjamin N Fry.   

Abstract

DNA-free RNA samples are essential to investigate gene regulation using real-time RT-PCR. This study evaluated eight RNA isolation methods in combination with TURBO DNase treatment and acid phenol extractions for their ability to produce DNA-free RNA from Campylobacter jejuni strains.

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Year:  2006        PMID: 17084472     DOI: 10.1016/j.mimet.2006.09.002

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  5 in total

1.  Full-Length gene enrichment by using an optimized RNA isolation protocol in Bixa orellana recalcitrant tissues.

Authors:  N L Rodríguez-Avila; J A Narváez-Zapata; M L Aguilar-Espinosa; R Rivera-Madrid
Journal:  Mol Biotechnol       Date:  2008-12-20       Impact factor: 2.695

2.  Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria.

Authors:  Fernando Lopes Pinto; Anders Thapper; Wolfgang Sontheim; Peter Lindblad
Journal:  BMC Mol Biol       Date:  2009-08-07       Impact factor: 2.946

3.  Purification and characterisation of dsRNA using ion pair reverse phase chromatography and mass spectrometry.

Authors:  Alison O Nwokeoji; An-Wen Kung; Peter M Kilby; David E Portwood; Mark J Dickman
Journal:  J Chromatogr A       Date:  2016-12-21       Impact factor: 4.759

4.  Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment.

Authors:  Csilla Paska; Imre Barta; Orsolya Drozdovszky; Balazs Antus
Journal:  PLoS One       Date:  2019-03-28       Impact factor: 3.240

5.  Transcriptomic studies and assessment of Yersinia pestis reference genes in various conditions.

Authors:  Lionel Koch; Thomas Poyot; Marine Schnetterle; Sophie Guillier; Estelle Soulé; Flora Nolent; Olivier Gorgé; Fabienne Neulat-Ripoll; Eric Valade; Florent Sebbane; Fabrice Biot
Journal:  Sci Rep       Date:  2019-02-21       Impact factor: 4.379

  5 in total

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