Literature DB >> 17077537

Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN.

Warude Dnyaneshwar1, Chavan Preeti, Joshi Kalpana, Patwardhan Bhushan.   

Abstract

Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon. The SCAR marker was found useful for identification of P. emblica in its commercial samples and Triphalachurna, a multi-component Ayurvedic formulation.

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Year:  2006        PMID: 17077537     DOI: 10.1248/bpb.29.2313

Source DB:  PubMed          Journal:  Biol Pharm Bull        ISSN: 0918-6158            Impact factor:   2.233


  11 in total

1.  SCAR makers and multiplex PCR-based rapid molecular typing of Lentinula edodes strains.

Authors:  Xueqian Wu; Haibo Li; Weiwei Zhao; Lizhong Fu; Huazheng Peng; Liang He; Junwen Cheng; Hailong Wei; Qingqi Wu
Journal:  Curr Microbiol       Date:  2010-04-01       Impact factor: 2.188

Review 2.  Molecular markers: a potential resource for ginger genetic diversity studies.

Authors:  Nor Asiah Ismail; M Y Rafii; T M M Mahmud; M M Hanafi; Gous Miah
Journal:  Mol Biol Rep       Date:  2016-09-01       Impact factor: 2.316

3.  Use of inter-simple sequence repeat markers to develop strain-specific SCAR markers for Flammulina velutipes.

Authors:  Hongyan Su; Lei Wang; Linde Liu; Xiaoyan Chi; Yuxiang Zhang
Journal:  J Appl Genet       Date:  2008       Impact factor: 3.240

4.  Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae).

Authors:  Kambiranda Devaiah; Subramani Paranthaman Balasubramani; Padma Venkatasubramanian
Journal:  Evid Based Complement Alternat Med       Date:  2011-02-20       Impact factor: 2.629

5.  Bamboo tea: reduction of taxonomic complexity and application of DNA diagnostics based on rbcL and matK sequence data.

Authors:  Thomas Horn; Annette Häser
Journal:  PeerJ       Date:  2016-12-08       Impact factor: 2.984

6.  Molecular cloning and development of RAPD-SCAR markers for Dimocarpus longan variety authentication.

Authors:  Luquan Yang; Shelly Fu; Md Asaduzzaman Khan; Weimin Zeng; Junjiang Fu
Journal:  Springerplus       Date:  2013-10-03

7.  Wound healing activity of topical application forms based on ayurveda.

Authors:  Hema Sharma Datta; Shankar Kumar Mitra; Bhushan Patwardhan
Journal:  Evid Based Complement Alternat Med       Date:  2011-05-26       Impact factor: 2.629

8.  SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha.

Authors:  Pramod Kumar Sairkar; Anjana Sharma; N P Shukla
Journal:  Mol Biol Int       Date:  2016-03-16

9.  The complete chloroplast genome sequence of Aconitum coreanum and Aconitum carmichaelii and comparative analysis with other Aconitum species.

Authors:  Inkyu Park; Wook-Jin Kim; Sungyu Yang; Sang-Min Yeo; Hulin Li; Byeong Cheol Moon
Journal:  PLoS One       Date:  2017-09-01       Impact factor: 3.240

10.  Development of a SCAR Marker-Based Diagnostic Method for the Detection of the Citrus Target Spot Pathogen Pseudofabraea citricarpa.

Authors:  Yuheng Yang; Junhua Hu; Fajing Chen; Dekuan Ding; Changyong Zhou
Journal:  Biomed Res Int       Date:  2018-06-03       Impact factor: 3.411

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