Literature DB >> 1706695

An improved technique for hyaluronan histochemistry using microwave irradiation.

S Hellström1, A Tengblad, C Johansson, U Hedlund, E Axelsson.   

Abstract

A hyaluronan binding protein (HABP), extracted from cartilage, was biotin-labelled and used for histochemical localization of hyaluronan (HA) in tissue sections. Various tissues were fixed for a mixture of formaldehyde and glutaraldehyde during microwave irradiation. The microwave oven when set at 700 W and 45 degrees C yielded an intense and specific staining of HA. Under these conditions the relative proportion of the two aldehydes did not influence the staining intensity. Aldehyde fixation during microwave irradiation for HA histochemistry, (1) save time, (2) eliminates the use of cetylpyridinium chloride (CPC), and (3) improves the reproducibility.

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Year:  1990        PMID： 1706695     DOI： 10.1007/bf01047452

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  16 in total

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Journal:  Stain Technol       Date:  1963-01

2.  Hyaluronan in experimental serous and purulent otitis media.

Authors:  C Laurent; S Hellström; A Tengblad; K Lilja
Journal:  Ann Otol Rhinol Laryngol       Date:  1989-09       Impact factor: 1.547

3.  Influence of cetylpyridinium chloride on the ultrastructural appearance of sulphated glycosaminoglycans in human colonic mucosa.

Authors:  F Malchiodi Albedi; A M Cassano; F Ciaralli; G Donelli; A Giuliani; P Mingazzini; V Marinozzi
Journal:  Histochemistry       Date:  1988

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Journal:  Histochemie       Date:  1965-10-01

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Authors:  W D Comper; T C Laurent
Journal:  Physiol Rev       Date:  1978-01       Impact factor: 37.312

6.  Histological fixation by microwave heating.

Authors:  C P Mayers
Journal:  J Clin Pathol       Date:  1970-04       Impact factor: 3.411

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Authors:  G R Login
Journal:  Am J Med Technol       Date:  1978-05

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Authors:  C E Kupchella; L Y Matsuoka; B Bryan; J Wortsman; J G Dietrich
Journal:  J Histochem Cytochem       Date:  1984-10       Impact factor: 2.479

Review 9.  Biochemistry of hyaluronan.

Authors:  T C Laurent
Journal:  Acta Otolaryngol Suppl       Date:  1987

10.  Fluorescent morphological probe for hyaluronate.

Authors:  C B Knudson; B P Toole
Journal:  J Cell Biol       Date:  1985-05       Impact factor: 10.539
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  9 in total

1.  Progression of bone marrow fibrosis in patients with essential thrombocythemia and polycythemia vera during anagrelide treatment.

Authors:  Magnus Hultdin; Gunnel Sundström; Anders Wahlin; Berith Lundström; Jan Samuelsson; Gunnar Birgegård; Anna Engström-Laurent
Journal:  Med Oncol       Date:  2007       Impact factor: 3.064

Review 2.  Tumor-associated hyaluronan. Providing an extracellular matrix that facilitates invasion.

Authors:  W Knudson
Journal:  Am J Pathol       Date:  1996-06       Impact factor: 4.307

3.  Microwave histoprocessing of bone marrow trephine biopsies.

Authors:  I Louw; D P De Beer; M J Du Plessis
Journal:  Histochem J       Date:  1994-06

4.  Localization of hyaluronan in various muscular tissues. A morphological study in the rat.

Authors:  C Laurent; G Johnson-Wells; S Hellström; A Engström-Laurent; A F Wells
Journal:  Cell Tissue Res       Date:  1991-02       Impact factor: 5.249

5.  An animal model for external otitis.

Authors:  P Emgård; S Hellström
Journal:  Eur Arch Otorhinolaryngol       Date:  1997       Impact factor: 2.503

6.  Bone marrow hyaluronan distribution in patients with acute myeloid leukemia.

Authors:  Gunnel Sundström; Inger Marie S Dahl; Magnus Hultdin; Berit Lundström; Anders Wahlin; Anna Engström-Laurent
Journal:  Med Oncol       Date:  2005       Impact factor: 3.064

7.  Effect of radiotherapy on expression of hyaluronan and EGFR and presence of mast cells in squamous cell carcinoma of the head and neck.

Authors:  Eva Lindell Jonsson; Karin Nylander; Lars Hallén; Göran Laurell
Journal:  Oncol Lett       Date:  2012-09-11       Impact factor: 2.967

8.  Localization and quantity of hyaluronan in urogenital organs of male and female rats.

Authors:  C Laurent; S Hellström; A Engström-Laurent; A F Wells; A Bergh
Journal:  Cell Tissue Res       Date:  1995-02       Impact factor: 5.249

9.  Identification of the two KIT isoforms and their expression status in canine hemangiosarcomas.

Authors:  Yi-Chen Chen; Jiunn-Wang Liao; Wei-Li Hsu; Shih-Chieh Chang
Journal:  BMC Vet Res       Date:  2016-07-16       Impact factor: 2.741
  9 in total

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