Genomic diversity in Mycobacterium avium: single nucleotide polymorphisms between the S and C strains of M. avium subsp. paratuberculosis and with M. a. avium.

Following identification of large genomic polymorphisms in a previous study, a polymerase chain reaction and sequencing strategy was used to identify single nucleotide polymorphisms (SNPs) in 25 genes in the sheep (S) and cattle (C) strains of Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) and between M. a. paratuberculosis and M. a. avium. From 12,117 bp of sequence representing 26 loci across 25 genes, 11 SNPs were identified between the S and C strains in eight genes: hsp65, sodA, dnaA, dnaN, recF, gyrB, inhA, and pks8. An in silico comparison of these M. a. paratuberculosis sequences and the M. a. avium 104 genome revealed 86 SNPs, which corresponded well with similar studies of SNPs in the M. avium complex.