| Literature DB >> 17045432 |
Zhong-Liang Jiang1, Qing-Wang Li, Wen-Ye Li, Jian-Hong Hu, Hong-Wei Zhao, Shu-Shan Zhang.
Abstract
A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing-thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (p<0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25 ml than in 0.5 ml types of straw (p<0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (p>0.05).Entities:
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Year: 2006 PMID： 17045432 DOI： 10.1016/j.anireprosci.2006.08.022
Source DB: PubMed Journal: Anim Reprod Sci ISSN： 0378-4320 Impact factor: 2.145