Literature DB >> 17040768

PCR detection of cryptosporidium: the way forward?

U M Morgan1, R C Thompson.   

Abstract

In this article, Una Morgan and Andrew Thompson briefly review the latest information on polymerase chain reaction (PCR)detection of Cryptosporidium parvum in both clinical and environmental samples. Current detection methods for Cryptosporidium are cumbersome, time-consuming and lack sensitivity. A variety of PCR tests have been described recently in the literature and this article discusses the advantages and disadvantages of each new technique and their potential for future diagnosis of Cryptosporidium.

Entities:  

Year:  1998        PMID: 17040768     DOI: 10.1016/s0169-4758(98)01247-2

Source DB:  PubMed          Journal:  Parasitol Today        ISSN: 0169-4758


  11 in total

1.  Comparison of diagnostic techniques for the detection of Cryptosporidium oocysts in animal samples.

Authors:  Marzieh Ezzaty Mirhashemi; Annetta Zintl; Tim Grant; Frances E Lucy; Grace Mulcahy; Theo De Waal
Journal:  Exp Parasitol       Date:  2015-02-03       Impact factor: 2.011

2.  Cryptosporidium spp. parasitize exotic birds that are commercialized in markets, commercial aviaries, and pet shops.

Authors:  Raquel Saucier Gomes; Franziska Huber; Sidnei da Silva; Teresa Cristina Bergamo do Bomfim
Journal:  Parasitol Res       Date:  2011-09-16       Impact factor: 2.289

3.  Detection of infectious Cryptosporidium parvum oocysts in surface and filter backwash water samples by immunomagnetic separation and integrated cell culture-PCR.

Authors:  G D Di Giovanni; F H Hashemi; N J Shaw; F A Abrams; M W LeChevallier; M Abbaszadegan
Journal:  Appl Environ Microbiol       Date:  1999-08       Impact factor: 4.792

4.  Comparison of PCR and microscopy for detection of Cryptosporidium parvum in human fecal specimens: clinical trial.

Authors:  U M Morgan; L Pallant; B W Dwyer; D A Forbes; G Rich; R C Thompson
Journal:  J Clin Microbiol       Date:  1998-04       Impact factor: 5.948

5.  A most-probable-number assay for enumeration of infectious Cryptosporidium parvum oocysts.

Authors:  T R Slifko; D E Huffman; J B Rose
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

Review 6.  New insights into human cryptosporidiosis.

Authors:  D P Clark
Journal:  Clin Microbiol Rev       Date:  1999-10       Impact factor: 26.132

7.  The first detection of Cryptosporidium deer-like genotype in cattle in Japan.

Authors:  Said Amer; Hajime Honma; Makoto Ikarashi; Ryu Oishi; Mikiko Endo; Kenichi Otawa; Yutaka Nakai
Journal:  Parasitol Res       Date:  2008-11-07       Impact factor: 2.289

8.  The sensitivity of PCR detection of Cryptosporidium oocysts in fecal samples using two DNA extraction methods.

Authors:  Gabriella Lindergard; Daryl V Nydam; Susan E Wade; Stephanie L Schaaf; Hussni O Mohammed
Journal:  Mol Diagn       Date:  2003

9.  Comparative efficacy of conventional primer sets in detection of Cryptosporidium parvum for diagnostic use.

Authors:  Sirri Kar; Arwid Daugschies; Berit Bangoura
Journal:  Parasitol Res       Date:  2010-01-28       Impact factor: 2.289

10.  Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran.

Authors:  Farid Tahvildar-Biderouni; Nima Salehi
Journal:  Gastroenterol Hepatol Bed Bench       Date:  2014
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