Literature DB >> 1704013

Detection and identification of Treponema hyodysenteriae by using oligodeoxynucleotide probes complementary to 16S rRNA.

N S Jensen1, T A Casey, T B Stanton.   

Abstract

Oligodeoxynucleotide probes (17 and 28 bases long) complementary to a unique region of Treponema hyodysenteriae 16S rRNA were developed. These probes bound specifically to partially purified rRNA and whole-cell rRNA of T. hyodysenteriae. No binding to partially purified rRNA or whole-cell rRNA of Treponema innocens, Treponema succinifaciens, Treponema bryantii, or Escherichia coli occurred under stringent conditions. The 28-base probe was 5 to 10 times more sensitive than the 17-base probe when hybridized with T. hyodysenteriae rRNA. The 28-base probe detected T. hyodysenteriae in the feces of experimentally inoculated pigs exhibiting clinical signs of swine dysentery.

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Year:  1990        PMID： 1704013      PMCID： PMC268261          DOI： 10.1128/jcm.28.12.2717-2721.1990

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

Review 1.  Diagnostic deoxyribonucleic acid probes for infectious diseases.

Authors:  F C Tenover
Journal:  Clin Microbiol Rev       Date:  1988-01       Impact factor: 26.132

2.  Oligonucleotide probes complementary to variable regions of ribosomal RNA discriminate between Mycoplasma species.

Authors:  U B Göbel; A Geiser; E J Stanbridge
Journal:  J Gen Microbiol       Date:  1987-07

3.  Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.

Authors:  S J Giovannoni; E F DeLong; G J Olsen; N R Pace
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490

4.  Use of an enzyme-linked immunosorbent assay for detection of Treponema hyodysenteriae infection in swine.

Authors:  J C Wright; G R Wilt; R B Reed; T A Powe
Journal:  J Clin Microbiol       Date:  1989-03       Impact factor: 5.948

5.  The production of dysentery in swine by feeding cultures containing a spirochaete.

Authors:  D J Taylor; T J Alexander
Journal:  Br Vet J       Date:  1971-11

6.  Improved selective medium for the isolation of Treponema hyodysenteriae.

Authors:  R A Kunkle; J M Kinyon
Journal:  J Clin Microbiol       Date:  1988-11       Impact factor: 5.948

7.  Species-specific oligonucleotide probes for rRNA of Clostridium difficile and related species.

Authors:  K H Wilson; R Blitchington; B Hindenach; R C Greene
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

8.  Erythrocytes as a source of essential lipids for Treponema hyodysenteriae.

Authors:  T B Stanton; C P Cornell
Journal:  Infect Immun       Date:  1987-02       Impact factor: 3.441

9.  Rapid identification of Campylobacter species using oligonucleotide probes to 16S ribosomal RNA.

Authors:  P J Romaniuk; T J Trust
Journal:  Mol Cell Probes       Date:  1989-06       Impact factor: 2.365

10.  New serotypes of Treponema hyodysenteriae.

Authors:  M E Mapother; L A Joens
Journal:  J Clin Microbiol       Date:  1985-08       Impact factor: 5.948
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  10 in total

1.  The phylogeny of intestinal porcine spirochetes (Serpulina species) based on sequence analysis of the 16S rRNA gene.

Authors:  B Pettersson; C Fellström; A Andersson; M Uhlén; A Gunnarsson; K E Johansson
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

2.  rRNA-based method for sensitive detection of Babesia bigemina in bovine blood.

Authors:  G R Reddy; J B Dame
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

3.  Production and characterization of monoclonal antibodies specific for lipooligosaccharide of Serpulina hyodysenteriae.

Authors:  R B Westerman; R M Phillips; L A Joens
Journal:  J Clin Microbiol       Date:  1995-08       Impact factor: 5.948

4.  Evaluation of the An-Ident system and an indole spot test for the rapid differentiation of porcine treponemes.

Authors:  M Bélanger; M Jacques
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

5.  Molecular characterization of Serpulina (Treponema) hyodysenteriae isolates representing serotypes 8 and 9.

Authors:  Z Li; N S Jensen; M Bélanger; M C L'Espérance; M Jacques
Journal:  J Clin Microbiol       Date:  1992-11       Impact factor: 5.948

6.  Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes.

Authors:  R T Marconi; L Lubke; W Hauglum; C F Garon
Journal:  J Clin Microbiol       Date:  1992-03       Impact factor: 5.948

7.  Detection of Mycoplasma hyopneumoniae by using rRNA-oligodeoxynucleotide hybridization.

Authors:  S Futo; Y Seto; S Mitsuse; Y Mori
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

8.  Characterization of two DNA probes specific for Serpulina hyodysenteriae.

Authors:  C Sotiropoulos; S C Smith; P J Coloe
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

9.  Characterization of Serpulina hyodysenteriae isolates of serotypes 8 and 9 from Quebec by restriction endonuclease fingerprinting and ribotyping.

Authors:  J Harel; M Bélanger; C Forget; M Jacques
Journal:  Can J Vet Res       Date:  1994-10       Impact factor: 1.310

10.  Rapid detection of Serpulina hyodysenteriae in diagnostic specimens by PCR.

Authors:  R O Elder; G E Duhamel; R W Schafer; M R Mathiesen; M Ramanathan
Journal:  J Clin Microbiol       Date:  1994-06       Impact factor: 5.948
  10 in total

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