Literature DB >> 17035495

Molecular method for identification of Rickettsia species in clinical and environmental samples.

Isabel Jado1, Raquel Escudero, Horacio Gil, María Isabel Jiménez-Alonso, Rita Sousa, Ana L García-Pérez, Manuela Rodríguez-Vargas, Bruno Lobo, Pedro Anda.   

Abstract

We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.

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Year:  2006        PMID： 17035495      PMCID： PMC1698399          DOI： 10.1128/JCM.01227-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

1.  Rickettsia africae, a tick-borne pathogen in travelers to sub-Saharan Africa.

Authors:  D Raoult; P E Fournier; F Fenollar; M Jensenius; T Prioe; J J de Pina; G Caruso; N Jones; H Laferl; J E Rosenblatt; T J Marrie
Journal:  N Engl J Med       Date:  2001-05-17       Impact factor: 91.245

2.  Comprehensive detection of bacterial populations by PCR amplification of the 16S-23S rRNA spacer region.

Authors:  Narjol González; Jaime Romero; Romilio T Espejo
Journal:  J Microbiol Methods       Date:  2003-10       Impact factor: 2.363

3.  Spotless rickettsiosis caused by Rickettsia slovaca and associated with Dermacentor ticks.

Authors:  Didier Raoult; Andras Lakos; Florence Fenollar; Jean Beytout; Philippe Brouqui; Pierre-Edouard Fournier
Journal:  Clin Infect Dis       Date:  2002-04-19       Impact factor: 9.079

4.  Rapid identification and differentiation of Bartonella species using a single-step PCR assay.

Authors:  W A Jensen; M Z Fall; J Rooney; D L Kordick; E B Breitschwerdt
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

5.  Comparison of PCR assays for detection of the agent of human granulocytic ehrlichiosis, Anaplasma phagocytophilum.

Authors:  Robert F Massung; Kimetha G Slater
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

6.  Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi.

Authors:  Ju Jiang; Teik-Chye Chan; Joseph J Temenak; Gregory A Dasch; Wei-Mei Ching; Allen L Richards
Journal:  Am J Trop Med Hyg       Date:  2004-04       Impact factor: 2.345

7.  Dermacentor-borne necrosis erythema and lymphadenopathy: clinical and epidemiological features of a new tick-borne disease.

Authors:  J A Oteo; V Ibarra; J R Blanco; V Martínez de Artola; F J Márquez; A Portillo; D Raoult; P Anda
Journal:  Clin Microbiol Infect       Date:  2004-04       Impact factor: 8.067

8.  Rickettsia parkeri: a newly recognized cause of spotted fever rickettsiosis in the United States.

Authors:  Christopher D Paddock; John W Sumner; James A Comer; Sherif R Zaki; Cynthia S Goldsmith; Jerome Goddard; Susan L F McLellan; Cynthia L Tamminga; Christopher A Ohl
Journal:  Clin Infect Dis       Date:  2004-03-01       Impact factor: 9.079

9.  First documented human Rickettsia aeschlimannii infection.

Authors:  Didier Raoult; Pierre-Edouard Fournier; Philippe Abboud; François Caron
Journal:  Emerg Infect Dis       Date:  2002-07       Impact factor: 6.883

10.  Coxiella burnetii genotyping.

Authors:  Olga Glazunova; Véronique Roux; Olga Freylikman; Zuzana Sekeyova; Ghislain Fournous; Judith Tyczka; Nikolai Tokarevich; Elena Kovacava; Thomas J Marrie; Didier Raoult
Journal:  Emerg Infect Dis       Date:  2005-08       Impact factor: 6.883
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  26 in total

1.  Molecular method for Bartonella species identification in clinical and environmental samples.

Authors:  Coral García-Esteban; Horacio Gil; Manuela Rodríguez-Vargas; Xeider Gerrikagoitia; Jesse Barandika; Raquel Escudero; Isabel Jado; Cristina García-Amil; Marta Barral; Ana L García-Pérez; Mangesh Bhide; Pedro Anda
Journal:  J Clin Microbiol       Date:  2007-12-19       Impact factor: 5.948

2.  Approaches for Reverse Line Blot-Based Detection of Microbial Pathogens in Ixodes ricinus Ticks Collected in Austria and Impact of the Chosen Method.

Authors:  Anna-Margarita Schötta; Michiel Wijnveld; Hannes Stockinger; Gerold Stanek
Journal:  Appl Environ Microbiol       Date:  2017-06-16       Impact factor: 4.792

3.  Rickettsia parkeri rickettsiosis in different ecological regions of Argentina and its association with Amblyomma tigrinum as a potential vector.

Authors:  Yamila Romer; Santiago Nava; Francisco Govedic; Gabriel Cicuttin; Amy M Denison; Joseph Singleton; Aubree J Kelly; Cecilia Y Kato; Christopher D Paddock
Journal:  Am J Trop Med Hyg       Date:  2014-10-27       Impact factor: 2.345

4.  Arthropod-Borne Bacteria Cause Nonmalarial Fever in Rural Ethiopia: A Cross-Sectional Study in 394 Patients.

Authors:  Jose M Ramos; Ramón Pérez-Tanoira; Inés Martín-Martín; Laura Prieto-Pérez; Abraham Tefasmariam; Gebre Tiziano; Raquel Escudero; Judit Gil-Zamorano; Horacio Gil-Gil; Miguel Górgolas; Isabel Jado
Journal:  Vector Borne Zoonotic Dis       Date:  2019-06-10       Impact factor: 2.133

5.  Infections and coinfections of questing Ixodes ricinus ticks by emerging zoonotic pathogens in Western Switzerland.

Authors:  Elena Lommano; Luce Bertaiola; Christèle Dupasquier; Lise Gern
Journal:  Appl Environ Microbiol       Date:  2012-04-20       Impact factor: 4.792

6.  Associations between coinfection prevalence of Borrelia lusitaniae, Anaplasma sp., and Rickettsia sp. in hard ticks feeding on reptile hosts.

Authors:  Radovan Václav; Martina Ficová; Pavol Prokop; Tatiana Betáková
Journal:  Microb Ecol       Date:  2010-08-14       Impact factor: 4.552

7.  Rickettsia spp., Ehrlichia sp. and Candidatus Midichloria sp. associated to ticks from a protected urban area in Buenos Aires City (Argentina).

Authors:  Gabriel L Cicuttin; María N De Salvo; José M Venzal; Santiago Nava
Journal:  Exp Appl Acarol       Date:  2022-01-13       Impact factor: 2.132

8.  Molecular identification of Rickettsia parkeri infecting Amblyomma triste ticks in an area of Argentina where cases of rickettsiosis were diagnosed.

Authors:  Gabriel Cicuttin; Santiago Nava
Journal:  Mem Inst Oswaldo Cruz       Date:  2013-02       Impact factor: 2.743

9.  Molecular method for discrimination between Francisella tularensis and Francisella-like endosymbionts.

Authors:  Raquel Escudero; A Toledo; Horacio Gil; Katarina Kovácsová; Manuela Rodríguez-Vargas; Isabel Jado; Cristina García-Amil; Bruno Lobo; Mangesh Bhide; Pedro Anda
Journal:  J Clin Microbiol       Date:  2008-07-23       Impact factor: 5.948

10.  Detection of Rickettsia in Rhipicephalus sanguineus ticks and Ctenocephalides felis fleas from southeastern Tunisia by reverse line blot assay.

Authors:  Fatma Khrouf; Youmna M'Ghirbi; Abir Znazen; Mounir Ben Jemaa; Adnene Hammami; Ali Bouattour
Journal:  J Clin Microbiol       Date:  2013-11-13       Impact factor: 5.948
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