Literature DB >> 17035493

Rapid simultaneous detection by real-time PCR of cytomegalovirus UL97 mutations in codons 460 and 520 conferring ganciclovir resistance.

Katharina Göhring1, Elfriede Mikeler, Gerhard Jahn, Klaus Hamprecht.   

Abstract

Ganciclovir (GCV) resistance is an emerging problem for transplant recipients. A sensitive and rapid real-time PCR approach for simultaneous and semiquantitative detection of human cytomegalovirus (HCMV) UL97 mutations in codons 460/520 was established by LightCycler and confirmed by restriction fragment length polymorphism and sequencing. Results from HCMV laboratory strains were compared with results from 11 GCV-resistant clinical isolates.

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Year:  2006        PMID： 17035493      PMCID： PMC1698385          DOI： 10.1128/JCM.01141-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

Review 1.  Antiviral drug resistance in human cytomegalovirus.

Authors:  S Chou
Journal:  Transpl Infect Dis       Date:  1999-06       Impact factor: 2.228

2.  Sensitivity of multiplex real-time PCR reactions, using the LightCycler and the ABI PRISM 7700 Sequence Detection System, is dependent on the concentration of the DNA polymerase.

Authors:  M M Exner; M A Lewinski
Journal:  Mol Cell Probes       Date:  2002-10       Impact factor: 2.365

3.  High impact of an expanded restriction fragment length polymorphism assay on detection of ganciclovir-resistant UL97 mutants of human cytomegalovirus.

Authors:  Tobias Eckle; Gerhard Jahn; Klaus Hamprecht
Journal:  Antimicrob Agents Chemother       Date:  2003-01       Impact factor: 5.191

4.  Rapid detection of codon 460 mutations in the UL97 gene of ganciclovir-resistant cytomegalovirus clinical isolates by real-time PCR using molecular beacons.

Authors:  Adrian C Yeo; Kwai Peng Chan; Gamini Kumarasinghe; Hui Kim Yap
Journal:  Mol Cell Probes       Date:  2005-08-10       Impact factor: 2.365

5.  Novel mutation in the UL97 gene of a clinical cytomegalovirus strain conferring resistance to ganciclovir.

Authors:  M N Hanson; L C Preheim; S Chou; C L Talarico; K K Biron; A Erice
Journal:  Antimicrob Agents Chemother       Date:  1995-05       Impact factor: 5.191

6.  Analysis of the UL97 phosphotransferase coding sequence in clinical cytomegalovirus isolates and identification of mutations conferring ganciclovir resistance.

Authors:  S Chou; A Erice; M C Jordan; G M Vercellotti; K R Michels; C L Talarico; S C Stanat; K K Biron
Journal:  J Infect Dis       Date:  1995-03       Impact factor: 5.226

7.  Ganciclovir resistance as a result of oral ganciclovir in a heart transplant recipient with multiple human cytomegalovirus strains in blood.

Authors:  F Baldanti; L Simoncini; A Sarasini; M Zavattoni; P Grossi; M G Revello; G Gerna
Journal:  Transplantation       Date:  1998-08-15       Impact factor: 4.939

8.  The influence of mixed HCMV UL97 wildtype and mutant strains on ganciclovir susceptibility in a cell associated plaque reduction assay.

Authors:  Tobias Eckle; Gerhard Jahn; Klaus Hamprecht
Journal:  J Clin Virol       Date:  2004-05       Impact factor: 3.168

Review 9.  Resistance of herpesviruses to antiviral drugs: clinical impacts and molecular mechanisms.

Authors:  Christian Gilbert; Julie Bestman-Smith; Guy Boivin
Journal:  Drug Resist Updat       Date:  2002-04       Impact factor: 18.500

10.  Ganciclovir-resistant cytomegalovirus disease after allogeneic stem cell transplantation: pitfalls of phenotypic diagnosis by in vitro selection of an UL97 mutant strain.

Authors:  Klaus Hamprecht; Tobias Eckle; Lothar Prix; Christoph Faul; Hermann Einsele; Gerhard Jahn
Journal:  J Infect Dis       Date:  2002-12-13       Impact factor: 5.226
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  7 in total

1.  Improved detection of mutated human cytomegalovirus UL97 by pyrosequencing.

Authors:  Birgit Schindele; Luise Apelt; Jörg Hofmann; Andreas Nitsche; Detlef Michel; Sebastian Voigt; Thomas Mertens; Bernhard Ehlers
Journal:  Antimicrob Agents Chemother       Date:  2010-09-27       Impact factor: 5.191

2.  Rapid identification of neuraminidase inhibitor resistance mutations in seasonal influenza virus A(H1N1), A(H1N1)2009, and A(H3N2) subtypes by melting point analysis.

Authors:  M Redlberger-Fritz; S W Aberle; R Strassl; T Popow-Kraupp
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-11-17       Impact factor: 3.267

3.  How I treat resistant cytomegalovirus infection in hematopoietic cell transplantation recipients.

Authors:  Firas El Chaer; Dimpy P Shah; Roy F Chemaly
Journal:  Blood       Date:  2016-10-19       Impact factor: 22.113

Review 4.  Antiviral drug resistance of human cytomegalovirus.

Authors:  Nell S Lurain; Sunwen Chou
Journal:  Clin Microbiol Rev       Date:  2010-10       Impact factor: 26.132

5.  Dynamics of the emergence of a human cytomegalovirus UL97 mutant strain conferring ganciclovir resistance in a pediatric stem-cell transplant recipient.

Authors:  Katharina Göhring; Tobias Feuchtinger; Elfriede Mikeler; Peter Lang; Gerhard Jahn; Rupert Handgretinger; Klaus Hamprecht
Journal:  J Mol Diagn       Date:  2009-05-28       Impact factor: 5.568

6.  Analysis of Ganciclovir-Resistant Cytomegalovirus Infection Caused by the UL97 Gene Mutation in Codons 460 and 520 in Pediatric Patients: A Case Series.

Authors:  Sophida Boonsathorn; Ekawat Pasomsub; Chonnamet Techasaensiri; Nopporn Apiwattanakul
Journal:  Open Forum Infect Dis       Date:  2019-11-04       Impact factor: 3.835

Review 7.  Antiviral Drug- and Multidrug Resistance in Cytomegalovirus Infected SCT Patients.

Authors:  Katharina Göhring; Klaus Hamprecht; Gerhard Jahn
Journal:  Comput Struct Biotechnol J       Date:  2015-02-10       Impact factor: 7.271
  7 in total

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