Literature DB >> 1703409

A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification.

F von Weizsäcker1, S Labeit, H K Koch, W Oehlert, W Gerok, H E Blum.   

Abstract

A simple and rapid method for the extraction, reverse transcription and PCR amplification of RNA from formalin-fixed, paraffin-embedded tissue is described. The procedure can be completed within 24 hours. In a first application of this method we detect human albumin mRNA in liver tissue, demonstrating the feasibility to retrospectively analyze gene expression and RNA viruses in fixed tissue.

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Year:  1991        PMID： 1703409     DOI： 10.1016/0006-291x(91)90502-x

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  16 in total

Review 1.  Effect of fixatives and tissue processing on the content and integrity of nucleic acids.

Authors:  Mythily Srinivasan; Daniel Sedmak; Scott Jewell
Journal:  Am J Pathol       Date:  2002-12       Impact factor: 4.307

2.  Optimizing gene expression analysis in archival brain tissue.

Authors:  Vivianna M D Van Deerlin; Lisa H Gill; Peter T Nelson
Journal:  Neurochem Res       Date:  2002-10       Impact factor: 3.996

3.  Analysis of estrogen receptor messenger RNA in breast carcinomas from archival specimens is predictive of tumor biology.

Authors:  C Carmeci; E C deConinck; T Lawton; D A Bloch; R J Weigel
Journal:  Am J Pathol       Date:  1997-05       Impact factor: 4.307

4.  Conversions of formaldehyde-modified 2'-deoxyadenosine 5'-monophosphate in conditions modeling formalin-fixed tissue dehydration.

Authors:  Vladimir K Rait; Qingrong Zhang; Daniele Fabris; Jeffrey T Mason; Timothy J O'Leary
Journal:  J Histochem Cytochem       Date:  2005-08-22       Impact factor: 2.479

5.  Assessment of the 2-d gel-based proteomics application of clinically archived formalin-fixed paraffin embedded tissues.

Authors:  Katarina Davalieva; Sanja Kiprijanovska; Momir Polenakovic
Journal:  Protein J       Date:  2014-04       Impact factor: 2.371

Review 6.  RNA expression analysis from formalin fixed paraffin embedded tissues.

Authors:  Susan M Farragher; Austin Tanney; Richard D Kennedy; D Paul Harkin
Journal:  Histochem Cell Biol       Date:  2008-08-05       Impact factor: 4.304

7.  DNA amplification by polymerase chain reaction from brain tissues embedded in paraffin.

Authors:  K Gall; J Pavelić; D Jadro-Santel; M Poljak; K Pavelić
Journal:  Int J Exp Pathol       Date:  1993-08       Impact factor: 1.925

8.  Diagnosis of DiGeorge syndrome in nuclei released from archival autoptic heart specimens using fluorescence in situ hybridization.

Authors:  G Calabrese; R Mingarelli; P Francalanci; R Boldrini; G Palka; C Bosman; G Novelli; B Dallapiccola
Journal:  Hum Genet       Date:  1996-04       Impact factor: 4.132

9.  Hepatitis B virus infection associated with hematopoietic tumors.

Authors:  E Galun; Y Ilan; N Livni; M Ketzinel; O Nahor; G Pizov; A Nagler; A Eid; A Rivkind; M Laster
Journal:  Am J Pathol       Date:  1994-11       Impact factor: 4.307

10.  Expression of the human Achaete-scute 1 gene in olfactory neuroblastoma (esthesioneuroblastoma).

Authors:  M E Carney; R C O'Reilly; B Sholevar; O I Buiakova; L D Lowry; W M Keane; F L Margolis; J L Rothstein
Journal:  J Neurooncol       Date:  1995-10       Impact factor: 4.130
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