| Literature DB >> 1703126 |
R R Kantor1, S L Giardina, A Bartolazzi, A J Townsend, C E Myers, K H Cowan, D L Longo, P G Natali.
Abstract
Mouse monoclonal antibodies (MAb) have been generated against the anionic isozyme of human glutathione S-transferase (GST pi). MAb AGST I can inhibit 50-70% of GST pi enzymatic activity and reacts with a 3-dimensional epitope which includes a putative glutathione binding site on GST pi. A sandwich enzyme-immunoassay established using MAb AGST I and a polyclonal antibody displayed a sensitivity of 0.5 ng/ml. Immunohistochemical analysis of human tissues demonstrated marked increases in GST pi levels in cancers of the brain, cervix, endometrium, colon, rectum and testis and in fibro- and chondrosarcomas.Entities:
Mesh：
Substances：
Year: 1991 PMID： 1703126 DOI： 10.1002/ijc.2910470206
Source DB: PubMed Journal: Int J Cancer ISSN： 0020-7136 Impact factor: 7.396