Literature DB >> 17011785

Purification, product characterization and kinetic properties of lipoxygenase from olive fruit (Olea europaea L.).

V Lorenzi1, J Maury, J Casanova, L Berti.   

Abstract

Lipoxygenase from olive fruit was purified to homogeneity for the first time after differential centrifugations and by hydrophobic chromatography. The enzyme had a molecular mass of 98 kDa and exhibited a maximal activity at pH 6. Lipoxygenase had a better affinity for linoleic acid (Km=82.44 microM) than for linolenic acid (Km = 306.26 microM). It is inhibited by linoleate:oxygen oxidoreductase (LOX) inhibitors like nordihydroguaiaretic acid (NDGA) or propyl gallate. The reaction product was 13-hydroperoxy octadecadienoic acid when linoleic acid was used as substrate.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 17011785     DOI： 10.1016/j.plaphy.2006.09.001

Source DB:  PubMed          Journal:  Plant Physiol Biochem        ISSN： 0981-9428            Impact factor:   4.270


  6 in total

1.  Two isoforms of lipoxygenase from mature grains of pearl millet [Pennisetum glaucum (L.) R. Br.]: purification and physico-chemico-kinetic characterization.

Authors:  Bunty Sharma; Laxman Kumar Chugh
Journal:  J Food Sci Technol       Date:  2017-03-27       Impact factor: 2.701

2.  The pepper 9-lipoxygenase gene CaLOX1 functions in defense and cell death responses to microbial pathogens.

Authors:  In Sun Hwang; Byung Kook Hwang
Journal:  Plant Physiol       Date:  2009-11-25       Impact factor: 8.340

3.  LOX Gene transcript accumulation in olive (Olea europaea L.) fruits at different stages of maturation: relationship between volatile compounds, environmental factors, and technological treatments for oil extraction.

Authors:  Innocenzo Muzzalupo; Barbara Macchione; Cristina Bucci; Francesca Stefanizzi; Enzo Perri; Adriana Chiappetta; Antonio Tagarelli; Giovanni Sindona
Journal:  ScientificWorldJournal       Date:  2012-05-01

4.  Purification and Product Characterization of Lipoxygenase from Opium Poppy Cultures (Papaver somniferum L.).

Authors:  Ivana Holková; Drahomíra Rauová; Michaela Mergová; Lýdia Bezáková; Peter Mikuš
Journal:  Molecules       Date:  2019-11-23       Impact factor: 4.411

5.  Olive Pomace-Derived Biomasses Fractionation through a Two-Step Extraction Based on the Use of Ultrasounds: Chemical Characteristics.

Authors:  María Del Mar Contreras; Irene Gómez-Cruz; Inmaculada Romero; Eulogio Castro
Journal:  Foods       Date:  2021-01-07

6.  HPLC Analysis and Biochemical Characterization of LOX from Eschscholtzia californica Cham.

Authors:  Renáta Kollárová; Ivana Holková; Drahomíra Rauová; Barbora Bálintová; Peter Mikuš; Marek Obložinský
Journal:  Molecules       Date:  2017-11-04       Impact factor: 4.411
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.