| Literature DB >> 17001096 |
M J Fogg1, P Alzari, M Bahar, I Bertini, J M Betton, W P Burmeister, C Cambillau, B Canard, M A Corrondo, M Carrondo, M Coll, S Daenke, O Dym, M P Egloff, F J Enguita, A Geerlof, A Haouz, T A Jones, Qingjun Ma, S N Manicka, M Migliardi, P Nordlund, R J Owens, Y Peleg, G Schneider, R Schnell, D I Stuart, N Tarbouriech, T Unge, A J Wilkinson, M Wilmanns, K S Wilson, O Zimhony, J M Grimes.
Abstract
The Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high-throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. Despite the challenging nature of some of these targets, 175 novel pathogen protein structures (approximately 220 including complexes) have been determined to date. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens.Entities:
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Year: 2006 PMID： 17001096 PMCID： PMC7161641 DOI： 10.1107/S0907444906030915
Source DB: PubMed Journal: Acta Crystallogr D Biol Crystallogr ISSN： 0907-4449